Division of Food Science and Culinary Arts, Shinhan University, 95, Hoam-ro, Uijeongbu-si, Gyeonggi, 11644, Republic of Korea.
Division of Food and Nutrition, Chonnam National University, 77 Yonbong-ro, Buk-gu, Gwangju, 61186, Republic of Korea.
Carbohydr Polym. 2019 Nov 15;224:115159. doi: 10.1016/j.carbpol.2019.115159. Epub 2019 Aug 10.
Silver nanoparticles (AgNPs) were prepared by reducing AgNO using biopolymer pullulan as both a reducing and stabilizing agent. The prepared AgNPs solution was blended with pectin to make active nanocomposite films. The formation of AgNPs in the solution was confirmed by characteristic surface plasmon resonance (SPR) peak of AgNPs at 400-500 nm, using UV-vis absorption spectroscopy. The prepared composite and nanocomposite films were characterized using UV, FE-SEM, and FT-IR. In addition, films color, optical, water contact angle, water vapor permeability, mechanical and antimicrobial properties were evaluated. FE-SEM analysis showed uniform distribution of AgNPs in the resulting nanocomposites films. The presence of AgNPs could affect the physical and mechanical properties of the prepared films. The color, moisture content, water vapor barrier properties, hydrophobicity, thickness, and elongation at break of the films were significantly increased after formation of composite with AgNPs, but tensile strength and elastic modulus of the films were decreased. FT-IR results indicated that AgNPs had good compatibility with biopolymers. In addition, nanocomposite films, especially pullulan/AgNPs and pullulan/pectin/AgNPs films exhibited better antimicrobial activity against food born pathogens, which suggests that prepared nanocomposite films can be used as active food packaging material to maintain food safety and to improve shelf life of the packaged food.
采用生物聚合物普鲁兰作为还原剂和稳定剂，通过还原硝酸银制备了银纳米粒子（AgNPs）。将制备的 AgNPs 溶液与果胶混合制成活性纳米复合膜。通过紫外可见吸收光谱，在 400-500nm 处确认了溶液中 AgNPs 的特征表面等离子体共振（SPR）峰，证明了 AgNPs 的形成。采用 UV、FE-SEM 和 FT-IR 对制备的复合和纳米复合膜进行了表征。此外，还评估了薄膜的颜色、光学、水接触角、水蒸气透过性、机械和抗菌性能。FE-SEM 分析表明，AgNPs 在所得纳米复合膜中均匀分布。AgNPs 的存在可能会影响制备薄膜的物理和机械性能。复合 AgNPs 后，薄膜的颜色、水分含量、水蒸气阻隔性能、疏水性、厚度和断裂伸长率显著增加，但拉伸强度和弹性模量降低。FT-IR 结果表明，AgNPs 与生物聚合物具有良好的相容性。此外，纳米复合膜，尤其是普鲁兰/AgNPs 和普鲁兰/果胶/AgNPs 膜对食源性病原体表现出更好的抗菌活性，这表明制备的纳米复合膜可用作活性食品包装材料，以维持食品安全并延长包装食品的保质期。
