Department of Food and Nutrition, BioNanocomposite Research Center, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Republic of Korea.
Department of Food and Nutrition, BioNanocomposite Research Center, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Republic of Korea.
Carbohydr Polym. 2019 Nov 15;224:115191. doi: 10.1016/j.carbpol.2019.115191. Epub 2019 Aug 12.
κ-Carrageenan-based (Carr) functional wound healing hydrogel films were prepared by incorporating chitosan capped sulfur nanoparticles (SNP) and grapefruit seed extract (GSE). In vivo wound healing effect of the hydrogel films was tested using artificially wounded male Sprague-Dawley rats. The hydrogel film (Carr/GSE/SNP) showed higher mechanical strength, swelling ratio and ultraviolet barrier properties than the carrageenan film, but the water vapor permeability and water solubility were decreased. The hydrogel film showed antibacterial activity strong enough to destroy Staphylococcus epidermis and Escherichia coli within 3 h of incubation and showed high biocompatibility against mouse fibroblast (L929 cell lines). The hydrogel film (Carr/GSE/SNP3%) showed excellent wound healing effect (1.3% wound area after 2 weeks) compared to the control group (31% wound area after 2 weeks) through in vivo wound healing test. Histological examination showed the complete appearance of the healed epidermis. The Carr/GSE/SNP hydrogel films are most likely to be used for the treatment of full-thickness wounds.
κ-卡拉胶基（Carr）功能性伤口愈合水凝胶膜是通过掺入壳聚糖封端的硫纳米粒子（SNP）和柚籽提取物（GSE）制备的。使用人工受伤的雄性 Sprague-Dawley 大鼠测试了水凝胶膜的体内伤口愈合效果。水凝胶膜（Carr/GSE/SNP）表现出比卡拉胶膜更高的机械强度、溶胀比和紫外线阻挡性能，但水蒸气透过率和水溶性降低。水凝胶膜表现出足够强的抗菌活性，可在孵育 3 小时内破坏表皮葡萄球菌和大肠杆菌，对小鼠成纤维细胞（L929 细胞系）表现出高生物相容性。通过体内伤口愈合试验，水凝胶膜（Carr/GSE/SNP3%）与对照组（2 周后 31%的伤口面积）相比，表现出优异的伤口愈合效果（2 周后 1.3%的伤口面积）。组织学检查显示愈合的表皮完全出现。Carr/GSE/SNP 水凝胶膜最有可能用于治疗全层伤口。
