Plant Genetic Engineering Laboratory, Department of Biotechnology, Bharathiar University, Coimbatore 641046, India.
Genomics Proteomics Bioinformatics. 2012 Dec;10(6):364-7. doi: 10.1016/j.gpb.2012.03.002. Epub 2012 Nov 29.
Boerhavia diffusa (B. diffusa), also known as Punarnava, is an indigenous plant in India and an important component in traditional Indian medicine. The accurate identification and collection of this medicinal herb is vital to enhance the drug's efficacy and biosafety. In this study, a DNA barcoding technique has been applied to identify and distinguish B. diffusa from its closely-related species. The phylogenetic analysis was carried out for the four species of Boerhavia using barcode candidates including nuclear ribosomal DNA regions ITS, ITS1, ITS2 and the chloroplast plastid gene psbA-trnH. Sequence alignment revealed 26% polymorphic sites in ITS, 30% in ITS1, 16% in ITS2 and 6% in psbA-trnH, respectively. Additionally, a phylogenetic tree was constructed for 15 species using ITS sequences which clearly distinguished B. diffusa from the other species. The ITS1 demonstrates a higher transition/transversion ratio, percentage of variation and pairwise distance which differentiate B. diffusa from other species of Boerhavia. Our study revealed that ITS and ITS1 could be used as potential candidate regions for identifying B. diffusa and for authenticating its herbal products.
猪殃殃（B. diffusa），又称印度芦子藤，是印度的本土植物，也是传统印度药物的重要组成部分。准确识别和采集这种药用植物对于提高药物的疗效和生物安全性至关重要。在本研究中，应用 DNA 条形码技术对猪殃殃与其近缘种进行鉴定和区分。对猪殃殃属的四个种进行了基于候选条形码序列的核核糖体 DNA 区 ITS、ITS1、ITS2 和叶绿体基因 psbA-trnH 的系统发育分析。序列比对显示 ITS 区有 26%的多态性位点，ITS1 区有 30%，ITS2 区有 16%，psbA-trnH 区有 6%。此外，使用 ITS 序列构建了 15 种的系统发育树，清晰地区分了猪殃殃和其他种。ITS1 区具有更高的转换/颠换比、变异百分比和种间成对距离，可将猪殃殃与其他猪殃殃种区分开来。本研究表明，ITS 和 ITS1 可作为鉴定猪殃殃及其草药产品的潜在候选区域。
