State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
R&D Center, JALA Group. Co., Shanghai 200233, China.
Bioresour Technol. 2019 Nov;292:122063. doi: 10.1016/j.biortech.2019.122063. Epub 2019 Aug 24.
In this study, Trichoderma harzianum LZ117 was obtained during screening of cellulolytic enzyme producers from samples collected in Tibet. T. harzianum LZ117 exhibits dramatically earlier enzyme induction (48 h) and shorter fermentation time (5 days) during cellulase production when compared with the widely studied strain T. reesei QM9414. Strain LZ117 showed 61% of the maximum cellulase activity at 48 h, whereas only 17% of the maximum cellulase activity was detected in QM9414 at the same culture time. Early induction and rapid production of cellulase were also observed in LZ117 when compared with two other T. harzianum strains. Significantly higher glucose yield was obtained using crude enzyme from strain LZ117 from pretreated corn stover and Jerusalem artichoke stalks when compared with that of T. harzianum reference strain K223452. These results indicate that strain LZ117 is a promising cellulase producer for bioconversion of lignocellulosic biomass.
在这项研究中，从西藏采集的样本中筛选出了纤维素酶产生菌，得到了哈茨木霉 LZ117。与广泛研究的里氏木霉 QM9414 相比，LZ117 在纤维素酶生产过程中表现出显著更早的酶诱导（48 小时）和更短的发酵时间（5 天）。LZ117 在 48 小时时表现出 61%的最大纤维素酶活性，而在相同的培养时间内，QM9414 仅检测到 17%的最大纤维素酶活性。与另外两种哈茨木霉菌株相比，LZ117 也表现出早期诱导和快速产生纤维素酶的特性。与哈茨木霉参考菌株 K223452 相比，LZ117 的粗酶在预处理的玉米秸秆和菊芋茎中获得了更高的葡萄糖产量。这些结果表明，LZ117 菌株是木质纤维素生物质生物转化的有前途的纤维素酶产生菌。
