Yang Jie, Reyes Loaiciga Cristopher, Yue Hou-Ru, Hou Ya-Jing, Li Jun, Li Cheng-Xi, Li Jing, Zou Yue, Zhao Shuai, Zhang Feng-Li, Zhao Xin-Qing
State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Research Center for Microbial and Enzyme Engineering Technology, College of Life Science and Technology, Guangxi University, Nanning 530004, China.
J Fungi (Basel). 2024 Oct 7;10(10):697. doi: 10.3390/jof10100697.
species have been reported as masters in producing cellulolytic enzymes for the biodegradation of lignocellulolytic biomass and biocontrol agents against plant pathogens and pests. In our previous study, a novel strain LZ117, which shows potent capability in cellulase production, was isolated. Herein, we conducted multilocus phylogenetic analyses based on DNA barcodes and performed time-scaled phylogenomic analyses using the whole genome sequences of the strain, annotated by integrating transcriptome data. Our results suggest that this strain represents a new species closely related to ( clade). Genes encoding carbohydrate-active enzymes (CAZymes), transporters, and secondary metabolites were annotated and predicted secretome in sp. LZ117 was also presented. Furthermore, genetic manipulation of this strain was successfully achieved using PEG-mediated protoplast transformation. A putative transporter gene encoding maltose permease (Mal1) was overexpressed, which proved that this transporter does not affect cellulase production. Moreover, overexpressing the native Cre1 homolog in LZ117 demonstrated a more pronounced impact of glucose-caused carbon catabolite repression (CCR), suggesting the importance of Cre1-mediated CCR in cellulase production of sp. LZ117. The results of this study will benefit further exploration of the strain LZ117 and related species for their applications in bioproduction.
有报道称某些物种是生产用于木质纤维素生物质生物降解的纤维素分解酶的高手，也是对抗植物病原体和害虫的生物防治剂。在我们之前的研究中，分离出了一种新型菌株LZ117，它在纤维素酶生产方面表现出强大的能力。在此，我们基于DNA条形码进行了多位点系统发育分析，并使用该菌株的全基因组序列进行了时间尺度的系统发育基因组分析，通过整合转录组数据对其进行注释。我们的结果表明，该菌株代表了一个与（进化枝）密切相关的新物种。对编码碳水化合物活性酶（CAZymes）、转运蛋白和次生代谢物的基因进行了注释，并预测了LZ117菌株的分泌组。此外，通过聚乙二醇介导的原生质体转化成功实现了对该菌株的基因操作。一个编码麦芽糖通透酶（Mal1）的假定转运蛋白基因被过表达，结果证明该转运蛋白不影响纤维素酶的产生。此外，在LZ117中过表达天然的Cre1同源物显示出葡萄糖引起的碳分解代谢物阻遏（CCR）有更明显的影响，这表明Cre1介导的CCR在LZ117菌株纤维素酶生产中的重要性。本研究结果将有助于进一步探索LZ117菌株及相关物种在生物生产中的应用。
