Department of Parasitology, Faculty of Veterinary Medicine, Ankara University, 06110 Ankara, Turkey.
Vet Parasitol. 2019 Sep;273:97-104. doi: 10.1016/j.vetpar.2019.08.013. Epub 2019 Aug 24.
This study aimed to investigate the presence of the natural transovarial transmission of tick-borne pathogens in unfed larvae obtained from engorged female ticks from domestic animals in Turkey. Larvae (n = 4530, 151 pools) obtained from 75 engorged female ticks and female carcasses were screened for the presence of certain tick-borne pathogens by PCR. The presence of transovarial transmission of Babesia occultans was detected in Hyalomma marginatum and Hy. excavatum, while Ba. ovis in Rhipicephalus bursa. Theileria annulata was detected only in Hy. excavatum and Rh. turanicus female carcasses, but not in their examined progenies. Additionally, Rickettsia aeschlimannii and Rickettsia raoultii were detected in Hy. marginatum and Dermacentor marginatus females, respectively, and all their examined larvae. Besides, Ri. slovaca was detected in a De. marginatus female carcass and its one of two examined larvae pools. The presence of mixed Ba. occultans and Ri. aeschlimannii infection was also determined in an Hy. marginatum female and its larvae. This is the first demonstration of transovarial transmission of Ba. occultans in naturally infected Hy. excavatum. These data suggested that Hy. excavatum may act as vector in the natural cycle of Ba. occultans.
本研究旨在调查在土耳其从家畜吸食血液的饱血雌性蜱中获得的未进食幼虫中是否存在蜱传病原体的自然经卵传递。通过 PCR 对来自 75 只饱血雌性蜱和雌性蜱尸的 4530 只幼虫（151 个池）进行了某些蜱传病原体的存在情况筛查。在硬蜱属和璃眼蜱属中检测到了边缘无形体的经卵传递，而在扇头蜱属中检测到了绵羊巴贝斯虫。环形泰勒虫仅在硬蜱属和土耳其斯坦革蜱的雌性蜱尸和未进食幼虫中被检测到，但在其检查的后代中未被检测到。此外，在边缘无形体和边缘革蜱雌性中分别检测到了拉氏泰勒虫和瑞氏泰勒虫，并且在所有检查的幼虫中都检测到了这两种病原体。此外，在一只边缘革蜱雌性蜱尸及其两个检查的幼虫池中检测到了斯洛伐克无形体。在一只硬蜱属雌性及其幼虫中还检测到了混合感染的边缘无形体和拉氏泰勒虫。这是首次在自然感染的璃眼蜱属中证实了边缘无形体的经卵传递。这些数据表明，硬蜱属可能在无形体的自然循环中充当媒介。
