Wang Ning, Ye Run-Ze, Yu Hui-Jun, Han Xiao-Yu, Tian Di, Gao Wan-Ying, Wang Bai-Hui, Du Li-Feng, Zhang Ming-Zhu, Shi Xiao-Yu, Zhu Dai-Yun, Shi Wenqiang, Jia Na, Jiang Jia-Fu, Sun Yi, Zhao Lin, Cui Xiao-Ming, Liu Zhi-Hong, Cao Wu-Chun
Institute of EcoHealth, School of Public Health, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250012, China.
State Key Laboratory of Pathogen and Biosecurity, AMMS, Beijing 100071, China.
iScience. 2025 Feb 21;28(3):112080. doi: 10.1016/j.isci.2025.112080. eCollection 2025 Mar 21.
, which emerged in Morocco in 1997, causes the Mediterranean spotted fever-like rickettsiosis in various Mediterranean countries and recently in Russia and China. Despite its increasing distribution, no available genome has been reported outside Morocco to date. Here, we isolated two strains of from (Ning-1 strain) and (Ning-2 strain) ticks in northwestern China and assembled their complete genomes. The genomes of the two strains were smaller than the Mediterranean MC16 strain, containing fewer pseudogenes, higher virulence factor coverage, and 154 unique orthogroups. The Ning-1 strain overwhelmed the Ning-2 strain with more obvious cytopathic effects, quicker growth, and faster plaque formation in cell culture, likely due to its unique gene, higher frequency of single nucleotide polymorphisms, and missense/silent ratio. The prevalence of was high among ticks in northwestern China. These findings highlight the genomic characteristics of and the necessity for enhanced surveillance of the emerging in the human population.
于1997年在摩洛哥出现，在各个地中海国家以及最近在俄罗斯和中国引发类似地中海斑疹热的立克次体病。尽管其分布范围不断扩大，但迄今为止在摩洛哥以外地区尚未有可用的基因组报道。在此，我们从中国西北部的 蜱（宁-1株）和 蜱（宁-2株）中分离出两株 ，并组装了它们的完整基因组。这两株菌的基因组比地中海MC16株小，假基因更少，毒力因子覆盖率更高，还有154个独特的直系同源群。在细胞培养中，宁-1株具有更明显的细胞病变效应、更快的生长速度和更快的噬斑形成，从而超过了宁-2株，这可能是由于其独特的 基因、更高的单核苷酸多态性频率以及错义/沉默比。在中国西北部的 蜱中， 的流行率很高。这些发现突出了 的基因组特征以及加强对人群中新兴 的监测的必要性。
