Johannes Christian, Obe Guenter
Faculty of Biology-Molecular Genetics, Center for Medical Biotechnology, University of Duisburg-Essen, 45117 Essen, Germany.
Methods Mol Biol. 2019;2031:121-134. doi: 10.1007/978-1-4939-9646-9_6.
Human peripheral lymphocytes (HPL) are non-cycling primary cells (G0 cells). They are easily collectable by venipuncture. In the presence of suitable culture media and stimulants in vitro HPL enter the cell cycle and divide mitotically. Metaphase-like stages can be arrested using the spindle fiber poison colcemid and prepared on microscopic slides. Following appropriate staining, chromosomal aberrations can be analyzed in the microscope. These aberrations may either be induced in vivo by environmental or occupational influences or in vitro after experimentally controlled manipulations in order to detect or to test the mutagenic potency of various agents.
人外周血淋巴细胞（HPL）是处于非循环状态的原代细胞（G0期细胞）。通过静脉穿刺很容易采集到它们。在体外合适的培养基和刺激物存在的情况下，HPL进入细胞周期并进行有丝分裂。可以使用纺锤体纤维毒物秋水仙酰胺使细胞停滞在类似中期的阶段，并制备在显微镜载玻片上。经过适当染色后，可在显微镜下分析染色体畸变。这些畸变既可以在体内由环境或职业影响诱发，也可以在体外经过实验控制的操作后诱发，以便检测或测试各种试剂的诱变能力。
