紫露草四分体细胞微核试验

Micronucleus Assay with Tetrad Cells of Tradescantia.

作者信息

Mišík Miroslav, Pichler Clemens, Rainer Bernhard, Nersesyan Armen, Mišíková Katarína, Knasmueller Siegfried

机构信息

Department of Internal Medicine I, Institute of Cancer Research, Medical University of Vienna, Vienna, Austria.

Department of Botany, Faculty of Natural Sciences, Comenius University of Bratislava, Bratislava, Slovakia.

出版信息

Methods Mol Biol. 2019;2031:325-335. doi: 10.1007/978-1-4939-9646-9_18.

DOI:10.1007/978-1-4939-9646-9_18

PMID:31473969

Abstract

The Tradescantia micronucleus assay has been used since 50 years for the detection of genotoxins (including carcinogens) in the environment. A large database concerning the effects of individual chemicals and complex environmental mixtures (soil, air and waters) has accumulated. In contrast to other mutagenicity test systems, the effects of low concentrations of heavy metals, radionuclides, certain herbicides, pesticides and gaseous mutagens can be detected and it is also possible to conduct in situ biomonitoring studies with plant. The test system has been validated and standardized protocols have been developed for laboratory experiments and for field studies which are described in this chapter.

摘要

自50年前以来，紫露草微核试验一直用于检测环境中的基因毒素（包括致癌物）。现已积累了一个关于单个化学品和复杂环境混合物（土壤、空气和水）影响的大型数据库。与其他致突变性测试系统不同，该试验能够检测低浓度重金属、放射性核素、某些除草剂、杀虫剂和气态诱变剂的影响，还能够利用植物进行原位生物监测研究。该测试系统已经过验证，本章将介绍针对实验室实验和实地研究制定的标准化方案。