Effects of Glucagon-Like Peptide-2-Expressing Not Different from Empty Vector.

() and glucagon-like peptide-2 (GLP-2) has been employed to improve weaned-animal's intestinal development. The goal of this study was to determine whether either exogenous or GLP-2 elicits the major effects on fecal microbiotas and cytokine responses in weaned-piglets. Ninety-six piglets weaned at 26 days were assigned to one of four groups: 1) Basal diet (Control), 2) empty vector-harboring (EV-SC), 3) GLP-2-expressing (GLP2-SC), and 4) recombinant human GLP-2 (rh-GLP2). At the start of the post-weaning period (day 0), and at day 28, fecal samples were collected to assess the bacterial communities via sequencing the V1-V2 region of the 16S-rRNA gene, and piglets' blood was also sampled to measure cytokine responses (., IL-1β, TNF-α, and IFN-γ). Revealed in this study, on the one hand, although supplementation did not significantly alter the growth of weaned-piglets, it exhibited the increases in the relative abundances of two core genera ( and ) and the decreases in the relative abundances of other two core genera ( and ) and cytokine levels (IL-1β and TNF-α) ( < 0.05, Control vs EV-SC; < 0.05, rh-GLP2 vs GLP2-SC). On the other hand, GLP-2 supplementation had no significant influence on fecal bacterial communities and cytokine levels, but it had better body weight and average daily gain ( < 0.05, Control vs EV-SC; < 0.05, rh-GLP2 vs GLP2-SC). Herein, altered the fecal microbiotas and cytokine response effects in weaned-piglets was due to rather than GLP-2.