Department of Nutrition and Movement Sciences, Nutrition and Toxicology Research Institute - School for Nutrition and Translational Research in Metabolism, Maastricht University Medical Center (MUMC+), Maastricht, The Netherlands.
Laboratory of Food Quality and Nutrition, Food Science and Technology Study Program, Department of Agricultural Product Technology, Faculty of Agricultural Technology, Brawijaya University, Malang-East Java, Indonesia.
Br J Nutr. 2019 Aug 14;122(3):241-251. doi: 10.1017/S0007114519001260.
For the same BMI, South Asians have a higher body fat percentage than Caucasians. There might be differences in the fatty acid (FA) handling in adipose tissue when both ethnicities are exposed to high-fat overfeeding. The objective of the present study was to investigate the molecular adaptation in relation to FA metabolism in response to overfeeding with a high-fat diet (OHFD) in South Asian and Caucasian men. Ten South Asian men (BMI 18-29 kg/m2) and ten Caucasian men (BMI 22-33 kg/m2), matched for body fat percentage, aged 20-40 years were included. A weight-maintenance diet (30 % fat, 55 % carbohydrate and 15 % protein) was given for 3 d followed by 3 d of overfeeding (150 % energy requirement) with a high-fat diet (60 % fat, 25 % carbohydrate and 15 % protein) while staying in a respiration chamber. Before and after overfeeding, abdominal subcutaneous fat biopsies were taken. Proteins were isolated, analysed and quantified for short-chain 3-hydroxyacyl-CoA dehydrogenase (HADH), carnitine palmitoyl-transferase 1α (CPT1a), adipose TAG lipase, perilipin A (PLINA), perilipin B, lipoprotein lipase and fatty acid binding protein 4 using Western blotting. OHFD decreased the HADH level (P < 0·05) in Caucasians more than in Asians (P < 0·05), but the baseline and after intervention HADH level was relatively higher in Caucasians. The level of CPT1a decreased in South Asians and increased in Caucasians (P < 0·05). PLINA did not change with diet but the level was higher in South Asians (P < 0·05). The observed differences in HADH and PLINA levels as well as in CPT1a response may be important for differences in the long-term regulation of energy (fat) metabolism in these populations.
对于相同的 BMI，南亚人比高加索人有更高的体脂百分比。当这两个种族都暴露在高脂肪过量喂养中时，脂肪组织中的脂肪酸（FA）处理可能存在差异。本研究的目的是研究与高脂肪饮食（OHFD）过量喂养相关的 FA 代谢的分子适应，以观察南亚人和高加索人男性的变化。纳入了 10 名南亚男性（BMI 18-29 kg/m2）和 10 名高加索男性（BMI 22-33 kg/m2），他们的体脂百分比相匹配，年龄在 20-40 岁之间。在呼吸室内，先让他们连续 3 天食用维持体重的饮食（30%脂肪、55%碳水化合物和 15%蛋白质），然后再连续 3 天食用高脂肪饮食（60%脂肪、25%碳水化合物和 15%蛋白质）进行过量喂养（能量需求的 150%）。在过量喂养前后，从腹部皮下脂肪活检中取出蛋白质。使用 Western 印迹法分析和定量短链 3-羟基酰基辅酶 A 脱氢酶（HADH）、肉碱棕榈酰转移酶 1α（CPT1a）、脂肪组织三酰基甘油脂肪酶、脂联素 A（PLINA）、脂联素 B、脂蛋白脂肪酶和脂肪酸结合蛋白 4 的蛋白质。OHFD 使高加索人的 HADH 水平降低（P<0.05）比南亚人更明显（P<0.05），但高加索人的基线和干预后的 HADH 水平相对较高。CPT1a 在南亚人和高加索人中都有所下降（P<0.05）。PLINA 没有随饮食而变化，但南亚人的水平较高（P<0.05）。HADH 和 PLINA 水平以及 CPT1a 反应的差异可能对这些人群中能量（脂肪）代谢的长期调节有重要意义。
