Yadegari Mehdi, Riahy Simin, Mirdar Shadmehr, Hamidian Gholamreza, Afkhami Seyed Mohsen, Saeidi Ayoub, Rhibi Fatma, Ben Abderrahman Abderraouf, Hackney Anthony C, Zouhal Hassane
Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Mazandaran, Mazandaran, Iran.
Faculty of Aerospace Medicine and subsurface, Army Medical University, Tehran, Iran.
Adv Respir Med. 2019;87(4):226-234. doi: 10.5603/ARM.2019.0037.
Evidence suggests that hypoxia and high-intensity exercise training can increase apoptosis of lung cells and Adiantum capillus-veneris (Ac-v) extract can have anti-apoptotic effects. Thus, the aim of the present study was to investigate the effect of chronic hypoxia and the (Ac-v) extraction as a supplement on TNF-a and P53 protein expression as well as the respiratory surface.
24 healthy Wistar rats (age = 4 weeks, weight = 72 = 9 gr) were trained using interval training for 6 weeks followed by a 3-week stay in hypoxia conditions. Half of the hypoxia samples received 500 ml/gr/per body weight daily (Ac-v) within 3 weeks of hypoxia. At the end, the lung tissue was removed for histological and immunohistological analysis.
After 3 weeks of hypoxia exposure following 6 weeks of exercise, expression of P53 and TNF-a increased and the respiratory surface decreased (p ≤ 0.05). After 3 weeks of taking the Ac-v extract during hypoxia exposure, reduced P53 and TNF-a expression and the increased respiratory surface were observed (p ≤ 0.05).
Chronic hypoxia may be considered as a strong stimulus leading to the expression of proteins involved in apoptosis and tissue disruption. However, our findings suggest that the antioxidative properties of Ac-v extract could decrease the destructive structural and molecular events that happen along with hypoxia exposure or intense exercise training.
有证据表明，缺氧和高强度运动训练可增加肺细胞凋亡，而铁线蕨提取物可能具有抗凋亡作用。因此，本研究旨在探讨慢性缺氧以及补充铁线蕨提取物对肿瘤坏死因子-α（TNF-α）和P53蛋白表达以及呼吸表面的影响。
选用24只健康的Wistar大鼠（4周龄，体重72±9克），采用间歇训练法训练6周，随后在缺氧条件下饲养3周。在缺氧的3周内，一半缺氧样本每天按500毫升/克体重的剂量给予铁线蕨提取物。最后，取出肺组织进行组织学和免疫组织学分析。
运动6周后进行3周缺氧暴露，P53和TNF-α的表达增加，呼吸表面减少（p≤0.05）。在缺氧暴露期间服用铁线蕨提取物3周后，观察到P53和TNF-α表达降低，呼吸表面增加（p≤0.05）。
慢性缺氧可被视为导致参与细胞凋亡和组织破坏的蛋白质表达的强烈刺激因素。然而，我们的研究结果表明，铁线蕨提取物的抗氧化特性可减少与缺氧暴露或高强度运动训练相关的破坏性结构和分子事件。
