Freie Universität Berlin, Institute of Chemistry and Biochemistry, Takustr. 3, 14195 Berlin, Germany.
Institut für Physik, Martin-Luther-Universität Halle-Wittenberg, Von-Danckelmann-Platz 3, 06120 Halle (Saale), Germany.
J Control Release. 2019 Oct;311-312:147-161. doi: 10.1016/j.jconrel.2019.08.035. Epub 2019 Aug 30.
Versatile, multifunctional nanomaterials for theranostic approaches in cancer treatment are highly on demand in order to increase therapeutic outcomes. Here, we developed thermo-responsive nanogels equipped with the efficient near-infrared (NIR) transducing polymer polypyrrole (PPY) for combinational photothermal and chemotherapeutic therapy along with photoacoustic imaging ability. Long-term stability and water-dispersibility of PPY was achieved using semi-interpenetration method for in situ polymerization of PPY into hydrophilic thermo-responsive nanogels. The semi-interpenetrated nanogels of spherical shape and with hydrodynamic sizes of around 200 nm retained the temperature response behaviour and exhibit excellent photothermal transducing abilities in the NIR region. The PPY nanogels served as photoacoustic contrast agents, which allowed determination of biodistribution profiles ex vivo. In addition, we developed a new method for biodistribution determination based on the photothermal response of the nanogels with an accuracy down to 12.5 μg/mL. We examined the ability of the nanogels as photothermal agents and drug delivery systems in vitro and in vivo. We showed that they efficiently inhibit tumor growth with combinational effects of chemotherapeutics and photothermal treatment. Our work encourages further exploration of nanogels as functional stabilizing matrix for photothermal transducers and their application as drug delivery devices in combination with photothermal therapy and imaging.
为了提高治疗效果，人们对用于癌症治疗的多功能、多用途治疗诊断一体化纳米材料的需求很高。在这里，我们开发了一种温敏纳米凝胶，它配备了高效的近红外（NIR）转换聚合物聚吡咯（PPY），用于联合光热和化学治疗以及光声成像能力。通过半互穿方法实现了 PPY 的长期稳定性和水分散性，即将 PPY 原位聚合到亲水性温敏纳米凝胶中。半互穿纳米凝胶呈球形，水动力尺寸约为 200nm，保留了温度响应行为，并在近红外区域表现出优异的光热转换能力。PPY 纳米凝胶可用作光声对比剂，允许在体外确定其生物分布曲线。此外，我们开发了一种新的基于纳米凝胶光热响应的生物分布测定方法，其准确度可达 12.5μg/mL。我们在体外和体内研究了纳米凝胶作为光热剂和药物输送系统的能力。我们表明，它们通过化学治疗和光热治疗的联合作用，有效地抑制了肿瘤的生长。我们的工作鼓励进一步探索纳米凝胶作为光热换能器的功能稳定基质，并将其作为药物输送装置与光热治疗和成像相结合的应用。
