College of Food Science and Engineering, Ocean University of China, No. 5, Yu Shan Road, Qingdao, Shandong Province 266003, China.
College of Food Science and Engineering, Ocean University of China, No. 5, Yu Shan Road, Qingdao, Shandong Province 266003, China.
Food Chem. 2020 Jan 30;304:125425. doi: 10.1016/j.foodchem.2019.125425. Epub 2019 Aug 26.
Ceramide 2-aminoethylphosphonate (CAEP) is a type of phosphonosphingolipids with potential trophic activity. In this work, complicated CAEP species from different aquatic products were comprehensively identified and semi-quantified by utilizing normal phase liquid chromatography/Q-Exactive mass spectrometry (NPLC/Q-Exactive-MS). We elucidated the fragment schemes of CAEP molecules and found the presence of methylated CAEP (Me-CAEP) species. Remarkably, quantitative results revealed that Loligo chinensis had the highest CAEP content of 4.9 ± 0.4 mg/g dry weight and the most complex molecular species composition, whereas Asterias amurenis had the lowest CAEP content of 1.9 ± 0.6 mg/g dry weight. The most common molecule was CAEP (d19:3-16:0). Additionally, statistical analysis revealed that five aquatic products can be effectively distinguished from their CAEP species; thus, CAEP molecules can play an important role in identifying processed products from aquatic products.
神经酰胺 2-氨基乙基膦酸酯 (CAEP) 是一种具有潜在营养活性的磷酸神经鞘脂。本工作利用正相液相色谱/高分辨 Q-Exactive 质谱联用技术（NPLC/Q-Exactive-MS）对不同水产食品中的复杂 CAEP 进行了全面鉴定和半定量分析。阐明了 CAEP 分子的碎裂规律，发现了甲基化 CAEP（Me-CAEP）的存在。值得注意的是，定量结果表明，中国枪乌贼的 CAEP 含量最高，为 4.9±0.4mg/g 干重，且分子种类最复杂；而星突江鲽的 CAEP 含量最低，为 1.9±0.6mg/g 干重。最常见的分子是 CAEP（d19:3-16:0）。此外，统计分析表明，五种水产食品可以通过其 CAEP 种类有效区分，因此，CAEP 分子在识别水产加工产品方面可能发挥着重要作用。
