Laboratory of Technology of Marine Bioproducts, Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kitashirakawaoiwakecho, Sakyo-ku, Kyoto, 606-8502, Japan.
Innovation Center, Nippon Flour Mills Co., Ltd, 5-1-3 Midorigaoka, Atsugi, Kanagawa, 243-0041, Japan.
Sci Rep. 2020 Aug 17;10(1):13891. doi: 10.1038/s41598-020-70888-0.
Sphingolipids are one of the major components of cell membranes and are ubiquitous in eukaryotic organisms. Ceramide 2-aminoethylphosphonate (CAEP) of marine origin is a unique and abundant sphingophosphonolipid with a C-P bond. Although molluscs such as squids and bivalves, containing CAEP, are consumed globally, the dietary efficacy of CAEP is not understood. We investigated the efficacy of marine sphingophosphonolipids by studying the effect of dietary CAEP on the improvement of the skin barrier function in hairless mice fed a diet that induces severely dry-skin condition. The disrupted skin barrier functions such as an increase in the transepidermal water loss (TEWL), a decrease in the skin hydration index, and epidermal hyperplasia were restored by CEAP dietary supplementation. Correspondingly, dietary CAEP significantly increased the content of covalently bound ω-hydroxyceramide, and the expression of its biosynthesis-related genes in the skin. These effects of dietary CAEP mimic those of dietary plant glucosylceramide. The novel observations from this study show an enhancement in the skin barrier function by dietary CAEP and the effects could be contributed by the upregulation of covalently bound ω-hydroxyceramide synthesis in the skin.
鞘脂类是细胞膜的主要组成部分之一，存在于真核生物中。来源于海洋的神经酰胺 2-氨基乙基膦酸酯（CAEP）是一种独特且丰富的具有 C-P 键的鞘磷脂。虽然含有 CAEP 的鱿鱼和双壳类等软体动物在全球范围内被食用，但 CAEP 的饮食功效尚不清楚。我们通过研究饮食 CAEP 对改善因饮食诱导而导致严重干燥皮肤条件的无毛小鼠皮肤屏障功能的影响，来研究海洋鞘磷脂的功效。饮食 CAEP 的补充恢复了皮肤屏障功能的破坏，如经表皮水分流失（TEWL）增加、皮肤水合指数降低和表皮过度增生。相应地，饮食 CAEP 显著增加了皮肤中共价结合的 ω-羟基神经酰胺的含量，以及与其生物合成相关的基因的表达。这些饮食 CAEP 的作用与饮食植物神经酰胺葡萄糖苷的作用类似。本研究的新发现表明，饮食 CAEP 可增强皮肤屏障功能，其作用可能是通过皮肤中共价结合的 ω-羟基神经酰胺合成的上调来实现的。
