Yamashita Shinji, Yutani Wakaba, Sugimoto Maho, Miyashita Kazuo, Kinoshita Mikio
Department of Life and Food Sciences, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, Japan.
Center for Industry-University Collaboration, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, Japan.
Metabolites. 2025 Feb 21;15(3):147. doi: 10.3390/metabo15030147.
: Ceramide 2-aminoethylphosphonate (CAEP), a major sphingolipid class in mollusks, possesses unique structures that are not observed in other sphingolipids. CAEP has a carbon-phosphorus bond and unusual long-chain bases (LCBs). CAEP has been reported to exhibit nutritional functions, such as improving skin conditions and promoting cholesterol metabolism. Objectives: In this study, we investigated the role of dietary CAEP in the formation of aberrant crypt foci (ACF) and colon inflammation induced by 1,2-dimethylhydrazine (DMH) in mice. : Five-week-old female Bagg Albino/c mice were divided into four groups ( = 11), which were treated with the respective experimental diet and DMH intraperitoneal injection nine times for ten weeks. The results obtained after administering CAEP were compared with those obtained after administering sphingomyelin (SPM), which is a major sphingolipid in mammal-derived foods. : The predominant LCB in the octopus-extracted CAEP was determined as hexadeca-4-sphingenine. Dietary CAEP suppressed the formation of ACF, and egg yolk-derived dietary SPM exerted a higher suppressive effect on the formation of ACF. Additionally, dietary CAEP suppressed the DMH-decreased expressions of two inflammation-related cytokines in the colon mucosa, whereas dietary SPM normalized the expressions of two cytokines different from those suppressed by CAEP. : CAEP provides intestinal protection, with effects that differ from those of SPM. The polar head groups or LCBs in sphingolipids are important for determining their nutritional function in the intestine. The study findings contribute toward the understanding of the nutritional benefits of sphingolipids in daily diets or supplements in maintaining intestinal health.
神经酰胺2-氨基乙基膦酸酯（CAEP）是软体动物中主要的鞘脂类，具有其他鞘脂类中未观察到的独特结构。CAEP具有碳-磷键和不寻常的长链碱基（LCB）。据报道，CAEP具有营养功能，如改善皮肤状况和促进胆固醇代谢。目的：在本研究中，我们调查了饮食中CAEP在1,2-二甲基肼（DMH）诱导的小鼠异常隐窝灶（ACF）形成和结肠炎症中的作用。：将5周龄雌性Bagg白化病/c小鼠分为四组（每组n = 11），分别用相应的实验饮食和腹腔注射DMH九次，持续十周。将给予CAEP后获得的结果与给予鞘磷脂（SPM）后获得的结果进行比较，SPM是哺乳动物源性食物中的主要鞘脂。：章鱼提取的CAEP中主要的LCB被确定为十六碳-4-鞘氨醇。饮食中的CAEP抑制了ACF的形成，而蛋黄来源的饮食SPM对ACF的形成具有更高的抑制作用。此外，饮食中的CAEP抑制了结肠黏膜中两种炎症相关细胞因子表达的降低，而饮食中的SPM使两种细胞因子的表达正常化，这两种细胞因子与CAEP抑制的细胞因子不同。：CAEP提供肠道保护，其作用与SPM不同。鞘脂中的极性头部基团或LCB对确定它们在肠道中的营养功能很重要。该研究结果有助于理解鞘脂在日常饮食或补充剂中对维持肠道健康的营养益处。
