Beijing Advanced Innovation Center for Structural Biology, School of Medicine, Tsinghua University, Beijing 100084, China.
Institute of Biology, Westlake Institute for Advanced Study, School of Life Sciences, Westlake University, 18 Shilongshan Road, Hangzhou 310024, Zhejiang, China.
Curr Opin Struct Biol. 2019 Dec;59:124-133. doi: 10.1016/j.sbi.2019.07.010. Epub 2019 Aug 30.
The spliceosome executes eukaryotic precursor messenger RNA (pre-mRNA) splicing to remove noncoding introns through two sequential transesterification reactions, branching and exon ligation. The fidelity of this process is based on the recognition of the conserved sequences in the intron and dynamic compositional and structural rearrangement of this multi-megadalton machinery. Since atomic visualization of the splicing active site in an endogenous Schizosaccharomyces pombe spliceosome in 2015, high-resolution cryoelectron microscopy (cryo-EM) structures of other spliceosome intermediates began to uncover the molecular mechanism. Recent advances in the structural biology of the spliceosome make it clearer the mechanisms of its assembly, activation, disassembly and exon ligation. Together, these discrete structural images give rise to a molecular choreography of the spliceosome.
剪接体通过两个连续的转酯反应，分支和外显子连接，执行真核前体信使 RNA（pre-mRNA）剪接以去除非编码内含子。这个过程的保真度基于对内含子中保守序列的识别以及这个多兆道尔顿机器的动态组成和结构重排。自 2015 年在天然的酿酒酵母剪接体中原子可视化剪接活性位点以来，其他剪接体中间产物的高分辨率冷冻电镜（cryo-EM）结构开始揭示分子机制。剪接体的结构生物学的最新进展使其更清楚地了解其组装、激活、解体和外显子连接的机制。这些离散的结构图像共同构成了剪接体的分子舞蹈。
