Silva Marcos J, Gonçalves Carolina P, Galvão Kleber M, D'Alpino Paulo H P, Nascimento Fábio D
Universidade Anhanguera de São Paulo-UNIAN, Osasco, SP, Brazil.
Universidade de Araraquara, Núcleo de Pesquisa em Biotecnologia, Centro, Araraquara, SP, Brazil.
Eur J Dent. 2019 Jul;13(3):295-302. doi: 10.1055/s-0039-1693751. Epub 2019 Sep 2.
In this study, a collagen-rich biomembrane obtained from porcine -intestinal submucosa for application in guided bone regeneration was developed and characterized. Then, its biological and mechanical properties were compared with that of commercial products ( [Baumer], [Critéria], [Bionnovation], and [Technodry]).
The biomembrane was extracted from porcine intestinal submucosa. Scanning electron microscopy, spectroscopic dispersive energy, glycosaminoglycan quantification, and confocal microscopy by intrinsic fluorescence were used to evaluate the collagen structural patterns of the biomembrane. Mechanical tensile and deformation tests were also performed.
The results of the methods used for experimental membrane characterizations were compared with that obtained by the commercial membranes and statistically analyzed (significance of 5%).
The collagen-rich biomembrane developed also exhibited a more organized, less porous collagen fibril network, with the presence of glycosaminoglycans. The experimental biomembrane exhibited mechanical properties, tensile strength, and deformation behavior with improved average stress/strain when compared with other commercial membranes tested. Benefits also include a structured, flexible, and -bioresorbable characteristics scaffold.
The experimental collagen-rich membrane developed presents physical-chemical, molecular, and mechanical characteristics similar to or better than that of the commercial products tested, possibly allowing it to actively participating in the process of bone neoformation.
在本研究中，开发并表征了一种从猪小肠黏膜下层获得的富含胶原蛋白的生物膜，用于引导骨再生。然后，将其生物学和力学性能与市售产品（[鲍默]、[克里特里亚]、[生物创新]和[技术干燥]）进行比较。
从猪小肠黏膜下层提取生物膜。使用扫描电子显微镜、能谱色散、糖胺聚糖定量以及通过固有荧光进行的共聚焦显微镜来评估生物膜的胶原蛋白结构模式。还进行了机械拉伸和变形测试。
将用于实验膜表征的方法结果与市售膜获得的结果进行比较，并进行统计分析（显著性为5%）。
所开发的富含胶原蛋白的生物膜还表现出更有组织、孔隙较少的胶原纤维网络，并存在糖胺聚糖。与其他测试的市售膜相比，实验生物膜表现出力学性能、拉伸强度和变形行为，平均应力/应变有所改善。优点还包括具有结构化、柔韧性和生物可吸收特性的支架。
所开发的实验性富含胶原蛋白的膜呈现出与测试的市售产品相似或更好的物理化学、分子和力学特性，可能使其能够积极参与骨新形成过程。
