Synthesis and Characterizations of a Collagen-Rich Biomembrane with Potential for Tissue-Guided Regeneration.

OBJECTIVES

In this study, a collagen-rich biomembrane obtained from porcine -intestinal submucosa for application in guided bone regeneration was developed and characterized. Then, its biological and mechanical properties were compared with that of commercial products ( [Baumer], [Critéria], [Bionnovation], and [Technodry]).

MATERIALS AND METHODS

The biomembrane was extracted from porcine intestinal submucosa. Scanning electron microscopy, spectroscopic dispersive energy, glycosaminoglycan quantification, and confocal microscopy by intrinsic fluorescence were used to evaluate the collagen structural patterns of the biomembrane. Mechanical tensile and deformation tests were also performed.

STATISTICAL ANALYSIS

The results of the methods used for experimental membrane characterizations were compared with that obtained by the commercial membranes and statistically analyzed (significance of 5%).

RESULTS

The collagen-rich biomembrane developed also exhibited a more organized, less porous collagen fibril network, with the presence of glycosaminoglycans. The experimental biomembrane exhibited mechanical properties, tensile strength, and deformation behavior with improved average stress/strain when compared with other commercial membranes tested. Benefits also include a structured, flexible, and -bioresorbable characteristics scaffold.

CONCLUSIONS

The experimental collagen-rich membrane developed presents physical-chemical, molecular, and mechanical characteristics similar to or better than that of the commercial products tested, possibly allowing it to actively participating in the process of bone neoformation.