College of Veterinary Medicine, University of Kirkuk, Kirkuk, Iraq.
College of Education for Pure Science, University of Kirkuk, Department of Mathematics, Kirkuk, Iraq.
Anim Biotechnol. 2021 Feb;32(1):100-105. doi: 10.1080/10495398.2019.1658595. Epub 2019 Sep 2.
Nanotechnology is a fast-growing research technology. Nanoparticles have intensive scientific applications in many fields. Depending on the physical and chemical characteristics of a nanoparticle, it can be used either as a treatment agent to fight disease or as a delivery vehicle to transport the therapeutic drug to a specified biological organ, tissue, and cell. Cytotoxicity evaluation of nanoparticles is one of the primary concerns in clinical practices to avoid unpredicted or undesirable interactions that could worsen the case. Iron oxide nanoparticle (IONP) is the most utilized nanoparticle in medical fields for treatment, diagnostic, and imaging. This paper is designated to investigate the cytotoxicity of IONPs that decorated with Trans-Activator of Transcription (TAT) protein. WST-1 assay and flow cytometry were used to assess the cytotoxicity of TAT-IONPs, which showed no significant cytotoxic effect on mammalian breast cancer cells (MCF-7). Nanoparticles accumulation in the cell's cytoplasm was evaluated from TEM images by measuring the size of the endosome. The results indicate that TAT-IONPs can be used as a safe and non-toxic nanoplatform for targeted delivery at 50 µg/ml or less. Also, they present an approach by which the area of intracellular endosome can be assessed from the TEM images of fixed cells. In this study, the endosome size increased in a time-dependent manner.
纳米技术是一项快速发展的研究技术。纳米颗粒在许多领域的科学应用中具有很强的实用性。根据纳米颗粒的物理和化学特性，它既可以用作治疗剂来对抗疾病，也可以用作输送载体将治疗药物输送到特定的生物器官、组织和细胞。纳米颗粒的细胞毒性评估是临床实践中主要关注的问题之一，以避免不可预测或不理想的相互作用，从而使病情恶化。氧化铁纳米颗粒（IONP）是医疗领域中应用最广泛的纳米颗粒，可用于治疗、诊断和成像。本文旨在研究转染激活因子（TAT）蛋白修饰的 IONP 的细胞毒性。WST-1 检测法和流式细胞术用于评估 TAT-IONP 的细胞毒性，结果表明，TAT-IONP 对哺乳动物乳腺癌细胞（MCF-7）没有明显的细胞毒性作用。通过测量内体的大小，从 TEM 图像评估纳米颗粒在细胞细胞质中的积累。结果表明，TAT-IONP 可作为一种安全无毒的纳米平台，用于在 50μg/ml 或更低浓度下进行靶向递送。此外，它们提供了一种从固定细胞的 TEM 图像评估细胞内内体面积的方法。在本研究中，内体的大小随时间呈依赖性增加。
