AGH University of Science and Technology, Faculty of Physics and Applied Computer Science, Krakow, Poland.
AGH University of Science and Technology, Faculty of Physics and Applied Computer Science, Krakow, Poland.
Spectrochim Acta A Mol Biomol Spectrosc. 2020 Aug 5;236:118355. doi: 10.1016/j.saa.2020.118355. Epub 2020 Apr 13.
Iron oxide nanoparticles (IONPs) have biomedical and biotechnological applications in magnetic imaging, drug-delivery, magnetic separation and purification. The biocompatibility of such particles may be improved by covering them with coating. In presented paper the biochemical anomalies of liver and kidney occurring in animals exposed to d-mannitol-coated iron(III) oxide nanoparticles (M-IONPs) were examined with Fourier transform infrared (FTIR) microspectroscopy. The dose of IONPs used in the study was significantly lower than those used so far in other research. Liver and kidney tissue sections were analysed by chemical mapping of infrared absorption bands originating from proteins, lipids, compounds containing phosphate groups, cholesterol and cholesterol esters. Changes in content and/or structure of the selected biomolecules were evaluated by comparison of the results obtained for animals treated with M-IONPs with those from control group. Biochemical analysis of liver samples demonstrated a few M-IONPs induced anomalies in the organ, mostly concerning the relative content of the selected compounds. The biomolecular changes, following exposition to nanoparticles, were much more intense within the kidney tissue. Biochemical aberrations found in the organ samples indicated at increase of tissue density, anomalies in fatty acids structure as well as changes in relative content of lipids and proteins. The simultaneous accumulation of lipids, phosphate groups as well as cholesterol and cholesterol esters in kidneys of rats exposed to IONPs may indicate that the particles stimulated formation of lipid droplets within the organ.
氧化铁纳米粒子（IONPs）在医学成像、药物输送、磁性分离和纯化等生物医学和生物技术领域有应用。通过覆盖涂层可以提高此类颗粒的生物相容性。本文采用傅里叶变换红外（FTIR）微光谱技术，研究了暴露于 D-甘露醇包覆的三氧化二铁纳米粒子（M-IONPs）的动物肝脏和肾脏的生化异常。研究中使用的 IONPs 剂量明显低于迄今为止其他研究中使用的剂量。通过对源自蛋白质、脂质、含磷酸盐基团的化合物、胆固醇和胆固醇酯的红外吸收带的化学图谱分析，对肝脏和肾脏组织切片进行了分析。通过将用 M-IONPs 处理的动物的结果与对照组的结果进行比较，评估了所选生物分子的含量和/或结构变化。对肝脏样本的生化分析表明，这些器官存在少数 M-IONPs 诱导的异常，主要涉及所选化合物的相对含量。与组织相比，纳米颗粒暴露后在肾脏组织中引起的生物分子变化更为强烈。在器官样本中发现的生化异常表明组织密度增加、脂肪酸结构异常以及脂质和蛋白质的相对含量变化。在暴露于 IONPs 的大鼠肾脏中同时积累脂质、磷酸盐基团以及胆固醇和胆固醇酯可能表明这些颗粒刺激了器官内脂滴的形成。
