Huang Hsiao-Wen, Chen Bang-Yuan, Wang Chung-Yi
1Department of Animal Science and Technology, National Taiwan University, Taipei, 106 Taiwan.
2Department of Food Science, Fu Jen Catholic University, Taipei, 242 Taiwan.
J Food Sci Technol. 2019 Sep;56(9):3988-3997. doi: 10.1007/s13197-019-03867-7. Epub 2019 Jun 11.
The aim of this study was to use high hydrostatic pressure treatment to enhance the extraction efficiency of the active components from the fruiting bodies of , and compare with those obtained by shake and ultrasonic extraction methods. The conditions of high pressure extraction (HPE) at 600 MPa, a liquid/solid ratio of 40:1, and 3 min of treatment yielded triterpenoids and adenosine concentrations of 410.41 mg/100 mL and 0.47 mg/100 mL, respectively, which did not differ significantly from those with the two other treatments-shake extraction at 180 rpm for 8 h and ultrasonic extraction at 50 Hz for 60 min. The HPE extracts significantly attenuated reactive oxygen species, nitric oxide and prostaglandin E production in lipopolysaccharide-stimulated RAW 264.7 cells than shake extracts did. SEM micrographs revealed that high-pressure caused physical morphological damage to the mycelium of fruiting bodies, such as distortion and disruption of mycelial cells, and increased the mass-transfer effectiveness of the solvent and solute. HPE can be employed as an efficient extraction technique for production of bioactive ingredients that might have a potential application in food and related industries.
本研究的目的是利用高静水压处理提高某子实体中活性成分的提取效率，并与振荡提取法和超声提取法所得结果进行比较。在600兆帕的高压提取（HPE）条件下，液固比为40:1，处理3分钟，三萜类化合物和腺苷的浓度分别为410.41毫克/100毫升和0.47毫克/100毫升，这与其他两种处理方法（180转/分钟振荡提取8小时和50赫兹超声提取60分钟）所得结果无显著差异。与振荡提取物相比，HPE提取物能显著降低脂多糖刺激的RAW 264.7细胞中活性氧、一氧化氮和前列腺素E的产生。扫描电子显微镜图像显示，高压对该子实体的菌丝体造成了物理形态损伤，如菌丝体细胞的变形和破裂，并提高了溶剂和溶质的传质效率。HPE可作为一种高效提取技术，用于生产可能在食品及相关行业具有潜在应用价值的生物活性成分。
