Mechanical Engineering Department - MicroTech Lab., Universitat Politècnica de Catalunya, Colom 7-11 08222, Terrassa, Barcelona, Spain.
Lab Chip. 2019 Oct 7;19(19):3249-3260. doi: 10.1039/c9lc00690g. Epub 2019 Sep 3.
The blood typing test is mandatory in any transfusion, organ transplant, and pregnancy situation. There is a lack of point-of-care (POC) blood typing that could perform both direct and indirect methods using a single droplet of whole blood. This study presents a new methodology combining a passive microfluidic blood-plasma separator (BPS) and a blood typing detector for the very first time, leading to a stand-alone microchip which is capable of determining the blood group from both direct and indirect methods simultaneously. The proposed design separates blood cells from plasma by applying hydrodynamic forces imposed on them, which overcomes the clogging issue and consequently maximizes the volume of the extracted plasma. An axial migration effect across the main channel is responsible for collecting the plasma in plasma collector channels. The BPS novel design approached 12% yield of plasma with 100% purity in approximately 10 minutes. The portable BPS was designed and fabricated to perform ABO/Rh blood tests based on the detection of agglutination in both antigens of RBCs (direct) and antibodies of plasma (indirect). The differences between agglutinated and non-agglutinated samples were distinguishable by the naked eye and also validated by particle analysis of microscopic pictures. The results of this passive BPS in ABO/Rh blood grouping verified the quality and quantity of the extracted plasma in practical applications.
血型检测在任何输血、器官移植和妊娠情况下都是强制性的。目前缺乏一种即时检测（POC）方法，这种方法可以使用一滴全血同时进行直接和间接方法。本研究首次提出了一种新的方法，将被动微流控血液-血浆分离器（BPS）和血型检测器结合在一起，形成一个独立的微芯片，能够同时从直接和间接方法确定血型。该设计通过对血液施加流体力来分离血细胞和血浆，从而解决了堵塞问题，并最大限度地增加了提取的血浆量。轴向迁移效应穿过主通道，负责将血浆收集到血浆收集器通道中。BPS 的新型设计在大约 10 分钟内以 100%的纯度获得了 12%的血浆产量。该便携式 BPS 是为了根据 RBC 表面抗原（直接）和血浆中的抗体（间接）的凝集检测来执行 ABO/Rh 血型测试而设计和制造的。肉眼可以区分凝集和非凝集样本之间的差异，也可以通过显微镜图片的颗粒分析来验证。这种被动 BPS 在 ABO/Rh 血型分组中的结果验证了实际应用中提取的血浆的质量和数量。
