三唑连接 RNA 的双联体寡核苷酸。

Duplex-forming Oligonucleotide of Triazole-linked RNA.

机构信息

Department of Chemistry, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo, 113-0033, Japan.

Present address: Institute for Solid State Physics, The University of Tokyo, Kashiwanoha 5-1-5, Chiba, 277-8581, Japan.

出版信息

Chem Asian J. 2019 Oct 1;14(19):3380-3385. doi: 10.1002/asia.201901112. Epub 2019 Sep 10.

DOI:10.1002/asia.201901112

PMID:31478313

Abstract

An oligonucleotide of triazole-linked RNA ( RNA) was synthesized by performing consecutive copper-catalyzed azide-alkyne cycloaddition reactions for elongation. The reaction conditions that had been optimized for the synthesis of 3-mer RNA were found to be inappropriate for longer oligonucleotides, and the conditions were reoptimized for the solid-phase synthesis of an 11-mer RNA oligonucleotide. Duplex formation of the 11-mer RNA oligonucleotide was examined with the complementary oligonucleotide of natural RNA to reveal the effects of the 2'-OH groups on the duplex stability.

摘要

通过连续进行铜催化的叠氮化物-炔烃环加成反应来合成三唑连接的 RNA（RNA）寡核苷酸。已经针对 3-mer RNA 的合成优化的反应条件对于更长的寡核苷酸并不合适，因此针对固相合成 11- mer RNA 寡核苷酸对条件进行了重新优化。通过与天然 RNA 的互补寡核苷酸一起检查 11-mer RNA 寡核苷酸的双链体形成，揭示了 2'-OH 基团对双链体稳定性的影响。