Mayet W J, Bachmann M, Pfeifer K, Schröder H C, Müller W E, Gudat W, Korting G W, Meyer zum Büschenfelde K H
I. Medizinische Klink, Universität Mainz, FRG.
Eur J Clin Invest. 1988 Oct;18(5):465-71. doi: 10.1111/j.1365-2362.1988.tb01041.x.
Skin lesions, especially at areas exposed to sunlight, prove to be a major form of manifestation of diseases related to Ro-antibodies and neonatal-, 'ANA-negative-', and cutaneous types of lupus erythematosus. A monoclonal Ro-antibody established by our group reacts with a 60 kD polypeptide in extracts from human spleen, whereas in extracts from human epidermis the monoclonal Ro-antibody and a purified Ro-antibody from a monospecific serum of a patient with subacute cutaneous lupus erythematosus reacted with a 60 kD and a 48 kD protein. Performing immunofluorescence microscopy on HEp2-cells both antibodies showed a nuclear speckled staining pattern and a reaction with cytokeratin filaments. On skin biopsies obtained from the patient, predominantly the basal layers of the proliferative stratum germinativum demonstrated a high rate of immunofluorescence; antigen-expression seems to depend on sunlight exposure. From these data we assume that, especially in subacute cutaneous lupus erythematosus, cross-reaction of the Ro-antibody with one of the lower molecular keratins happens in that area of human epidermis which histologically shows cellular destruction.
皮肤病变，尤其是暴露于阳光下的部位，是与Ro抗体以及新生儿型、“抗核抗体阴性”型和皮肤型红斑狼疮相关疾病的主要表现形式。我们团队建立的一种单克隆Ro抗体与人类脾脏提取物中的一种60kD多肽发生反应，而在人类表皮提取物中，该单克隆Ro抗体和来自一名亚急性皮肤型红斑狼疮患者单特异性血清的纯化Ro抗体与一种60kD和一种48kD蛋白发生反应。在HEp2细胞上进行免疫荧光显微镜检查时，两种抗体均显示出核斑点状染色模式，并与细胞角蛋白丝发生反应。从该患者获取的皮肤活检标本中，主要是增殖性生发层的基底层显示出高免疫荧光率；抗原表达似乎取决于阳光照射。根据这些数据我们推测，尤其是在亚急性皮肤型红斑狼疮中，Ro抗体与一种分子量较低的角蛋白之一的交叉反应发生在人类表皮组织学显示细胞破坏的区域。
