High-performance-liquid-chromatographic analysis of radiolabelled prostaglandins and fatty acids with the radial compression column.

The method described is a rapid and reproducible separation of different prostanoids. With the use of a reversed phase 5 micron-radial compression column instead of a steel column-HPLC analysis is improved within a shorter time. In order to separate also the non-polar substances like arachidonic acid (AA) and hydroxy fatty acids in an appropriate time, a gradient-elution is used. The radioactivity is counted immediately after the column within a radioactivity monitor. With this system it is possible to examine the AA-metabolites enzymatically formed in cells or tissue from exogenous AA.