Eling T, Warnock R, Dick D, Tainer B
Prostaglandins Med. 1980 Nov;5(5):345-55. doi: 10.1016/0161-4630(80)90106-8.
Separation of the major metabolites of arachidonic acid (AA) produced by the cyclo-oxygenase and the lipoxygenases was achieved by using reverse phase high-pressure liquid chromatography. Prostaglandins (PGs), thromboxane B2 (TXB2), and AA were separated on a C-18 radial compression column. An initial isocratic elution resolved the PGs and TXB2 which was followed by a linear gradient in order to elute AA. Variations of the gradient elution shape were required to permit the separation of 12-L-hydroxy-5,8,10-heptadecatrienoic acid, 5-12 and 15-hydroxy-5,8,11,14-eicosatetraenoic acid. The recovery of the labeled AA and its metabolites was investigated. Use of these separation methods and radiolabeled substrates should permit investigators to obtain reproducibly in one chromatographic run adequate separation and quantitation of both PGs and hydroxy fatty acid systems.
利用反相高压液相色谱法实现了由环氧化酶和脂氧化酶产生的花生四烯酸（AA）主要代谢物的分离。前列腺素（PGs）、血栓素B2（TXB2）和AA在C-18径向压缩柱上进行分离。最初的等度洗脱分离出PGs和TXB2，随后进行线性梯度洗脱以洗脱AA。需要改变梯度洗脱形状以实现12-L-羟基-5,8,10-十七碳三烯酸、5-12和15-羟基-5,8,11,14-二十碳四烯酸的分离。研究了标记AA及其代谢物的回收率。使用这些分离方法和放射性标记底物应能使研究人员在一次色谱运行中可重复地获得PGs和羟基脂肪酸系统的充分分离和定量。
