UCEFF, SC, Brazil.
Faculdade São Leopoldo Mandic, Campinas, SP, Brazil.
J Photochem Photobiol B. 2019 Oct;199:111592. doi: 10.1016/j.jphotobiol.2019.111592. Epub 2019 Aug 14.
Current management of oral potentially malignant disorders is careful monitoring. Unfortunately, the 'watch and wait' approach only generates anxiety and a feeling of powerlessness, especially to those caring for patients. Photobiomodulation (PBM) has emerged as a potential strategy to inhibit possible transforming cells. The aim of this study was to investigate the effect of LED-based PBM on the progression of malignant invasion into a fibroblast-based stroma. An in vitro model of carcinoma in situ (CIS) containing stromal fibroblasts and carcinoma cells in co-culture was used to study the effect of PBM on the expansion of CIS colonies. A second model of co-culture (cells separated by membrane), was used to study cell counts, viability and apoptosis following PBM at high doses (36 J/cm). The data was analyzed using Kruskal-Wallis, Dunn's test and non-linear regression, wherever appropriate. PBM was able to inhibit the expansion of CIS colonies as well as the total number of colonies after 72 h of treatment (p < 0.05). Cell viability, apoptosis and death assays revealed an overall advantage of stromal fibroblasts over carcinoma cells after high-dose PBM. In conclusion, LED-based PBM at high doses inhibited the progression and number of oral squamous cell carcinoma colonies without affecting the surrounding stromal fibroblasts in vitro.
目前，口腔潜在恶性疾病的治疗方法是密切监测。不幸的是，“观望等待”的方法只会引起焦虑和无力感，尤其是对那些照顾患者的人来说。光生物调节（PBM）已成为抑制潜在转化细胞的一种潜在策略。本研究旨在探讨基于 LED 的 PBM 对恶性侵袭进入纤维母细胞基质的进展的影响。我们使用包含间质成纤维细胞和癌性细胞共培养的原位癌（CIS）的体外模型来研究 PBM 对 CIS 集落扩张的影响。我们还使用共培养的第二个模型（细胞通过膜隔开）来研究 PBM 后高剂量（36 J/cm）对细胞计数、活力和细胞凋亡的影响。在适当的情况下，使用 Kruskal-Wallis、Dunn 检验和非线性回归来分析数据。PBM 能够抑制 CIS 集落的扩张以及治疗 72 小时后总集落数量（p < 0.05）。细胞活力、细胞凋亡和死亡检测显示，高剂量 PBM 后，基质成纤维细胞总体上优于癌细胞。总之，基于 LED 的 PBM 在高剂量下可抑制口腔鳞状细胞癌集落的进展和数量，而不会影响体外周围的基质成纤维细胞。
