Computational Optics Laboratory, School of Science, Jiangnan University, Wuxi, Jiangsu, 214122, China.
Single Molecule Nanometry Laboratory (Sinmolab), Nanjing Agricultural University, Nanjing, 210095, China.
Biosens Bioelectron. 2019 Oct 15;143:111632. doi: 10.1016/j.bios.2019.111632. Epub 2019 Aug 28.
We present a sunlight based handheld smartphone spectrometer. The device first gathers the sunlight to pass through the sample, and then the transmitted light illuminates on a grating to generate spectrum finally recorded by the smartphone monochrome camera. All the optical elements are assembled with the smartphone to integrate a handheld device with the size of 140.2 mm × 67.4 mm × 80.5 mm. Besides, a smartphone application is also developed for automatic spectral calibration, detection, analysis and display. Compared to the white light emitting diode and the halogen lamp, the sunlight has more uniform distribution covering the entire visible spectral range; and the proposed device also avoids the bulky sizes of those broadband light sources. Additionally, the monochrome camera is used instead of the color camera not only to pursue a high spectral resolution as 0.276 nm/pixel but also to avoid the color overlapping. We demonstrate the device capability on detecting avian influenza virus H7N9 and porcine circovirus type 2 antibodies, proving the device has rather high sensitivity similar to the commercial microplate reader. Considering its advantages as compact size, high spectral resolution and detecting sensitivity, it is believed the proposed sunlight based handheld smartphone spectrometer is potential to be broadly applied in on-site detections.
我们提出了一种基于阳光的手持式智能手机光谱仪。该设备首先收集阳光，使其穿过样品，然后透过的光照射在光栅上，最终由智能手机单色相机记录光谱。所有光学元件都与智能手机组装在一起，集成了一款尺寸为 140.2mm×67.4mm×80.5mm 的手持式设备。此外，还开发了一个智能手机应用程序，用于自动进行光谱校准、检测、分析和显示。与白色发光二极管和卤素灯相比，阳光在整个可见光谱范围内具有更均匀的分布；并且所提出的设备还避免了那些宽带光源的庞大尺寸。此外，使用单色相机而不是彩色相机不仅可以追求 0.276nm/pixel 的高光谱分辨率，还可以避免颜色重叠。我们在检测禽流感病毒 H7N9 和猪圆环病毒 2 型抗体方面展示了该设备的性能，证明该设备具有与商用微孔板读数器相当的高灵敏度。考虑到其紧凑尺寸、高光谱分辨率和检测灵敏度的优势，相信这种基于阳光的手持式智能手机光谱仪具有广泛应用于现场检测的潜力。
