Zhuo Erhuan, Xia Huanxin, Hu Huan, Lin Yu
Zhejiang University-University of Illinois Urbana-Champaign Institute, Zhejiang University, Haining 314400, China.
Biosensors (Basel). 2024 Dec 3;14(12):590. doi: 10.3390/bios14120590.
Smartphones equipped with highly integrated sensors are increasingly being recognized as powerful tools for rapid on-site testing. Here, we propose a low-cost, portable, and highly multiplexed smartphone-based spectrometer capable of collecting three types of spectra-transmission, reflection, and fluorescence-by simply replacing the optical fiber attached to the housing. Spectral analysis is performed directly on the smartphone using a custom-developed app. Furthermore, we introduce a high signal-to-noise ratio (SNR) caffeine detection scheme that leverages aspirin and salicylic acid as fluorescent probes, allowing for the rapid and straightforward detection of caffeine in various samples. The fluorescence quenching of the probes was found to be linearly related to the caffeine concentration (0-200 μM), and the recoveries of the commercially available caffeine-containing samples were in the range of 98.0333-105.6000%, with a limit of detection (LOD) of 2.58 μM. The reliability and stability of the on-site assay using the smartphone spectrometer were verified. More importantly, this spectrometer demonstrates great potential as a versatile device for use outside of laboratory settings by enabling different operating modes tailored to various scenarios.
配备高度集成传感器的智能手机日益被视为快速现场检测的强大工具。在此，我们提出一种低成本、便携式且高度多路复用的基于智能手机的光谱仪，通过简单更换连接到外壳的光纤，该光谱仪能够收集三种类型的光谱——透射光谱、反射光谱和荧光光谱。使用定制开发的应用程序在智能手机上直接进行光谱分析。此外，我们引入了一种高信噪比（SNR）的咖啡因检测方案，该方案利用阿司匹林和水杨酸作为荧光探针，可快速直接地检测各种样品中的咖啡因。发现探针的荧光猝灭与咖啡因浓度（0 - 200 μM）呈线性相关，市售含咖啡因样品的回收率在98.0333 - 105.6000%范围内，检测限（LOD）为2.58 μM。使用智能手机光谱仪进行现场检测的可靠性和稳定性得到了验证。更重要的是，这种光谱仪通过针对不同场景启用不同的操作模式，展示了作为实验室外通用设备的巨大潜力。
