使用聚蔗糖-泛影葡胺梯度离心法富集自发形成花环细胞群和补体依赖形成花环细胞群。
Enrichment in spontaneous and complement dependent rosette forming cell populations using Ficoll-Hypaque gradient centrifugation.
作者信息
Carosella E D, Paulone I, Rohwedder R W, Bachmann A E
出版信息
Experientia. 1976 Nov 15;32(11):1484-6. doi: 10.1007/BF01937450.
PMID:791666
Abstract
Peripheral blood human T (TL) and B (BL) lymphocytes were separated by continuous Ficoll-Hypaque (FH) gradients. BL were found at the 1050 density interfase (70.5% EAC rosettes) with no TL (0% EAC rosettes); while at the 1068 density interfase there was an enrichment of TL (68% E rosettes) with very few BL (8.5% EAC rosettes).
摘要
通过连续的Ficoll-泛影葡胺（FH）梯度分离外周血人T（TL）淋巴细胞和B（BL）淋巴细胞。在密度为1050的界面处发现BL（EAC玫瑰花结形成率为70.5%），无TL（EAC玫瑰花结形成率为0%）；而在密度为1068的界面处，TL富集（E玫瑰花结形成率为68%），BL极少（EAC玫瑰花结形成率为8.5%）。
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