新型携带高水平替加环素耐药基因（X4）的质粒的完整核苷酸序列。
Complete Nucleotide Sequence of a Novel Plasmid Bearing the High-Level Tigecycline Resistance Gene (X4).
机构信息
National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, Guangzhou, China.
Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou, China.
出版信息
Antimicrob Agents Chemother. 2019 Oct 22;63(11). doi: 10.1128/AAC.01373-19. Print 2019 Nov.
Abstract
We reported the complete nucleotide sequence of a (X4)-carrying plasmid, pSTB20-1T, from a tigecycline-resistant isolate in China. Sequence analysis indicated that pSTB20-1T contains a hybrid plasmid backbone and a (X4)-containing multidrug resistance region, likely originated through recombination of multiple plasmids. (X4) was flanked by two IS, which may be responsible for (X4) mobilization. The occurrence and transmission of this novel hybrid plasmid may exacerbate the spread of the clinically significant (X4) gene.
摘要
我们报道了一株来自中国的替加环素耐药株中携带（X4）的质粒 pSTB20-1T 的完整核苷酸序列。序列分析表明，pSTB20-1T 含有混合质粒骨架和一个含有多种耐药基因的（X4）区域，可能是通过多个质粒的重组而产生的。（X4）两侧是两个 IS，它们可能负责（X4）的移动。这种新型混合质粒的出现和传播可能会加剧临床上重要的（X4）基因的传播。
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本文引用的文献
1
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Nat Microbiol. 2019 Sep;4(9):1457-1464. doi: 10.1038/s41564-019-0496-4. Epub 2019 Jun 24.
2
Emergence of plasmid-mediated high-level tigecycline resistance genes in animals and humans.动物和人类中质粒介导的高水平替加环素耐药基因的出现。
Nat Microbiol. 2019 Sep;4(9):1450-1456. doi: 10.1038/s41564-019-0445-2. Epub 2019 May 27.
3
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Appl Environ Microbiol. 2018 Mar 1;84(6). doi: 10.1128/AEM.02430-17. Print 2018 Mar 15.
4
Plasmids of Diverse Inc Groups Disseminate the Fosfomycin Resistance Gene among Escherichia coli Isolates from Pigs, Chickens, and Dairy Cows in Northeast China.不同 Inc 群质粒在中国东北猪、鸡和奶牛源大肠杆菌中的传播与fosfomycin 耐药基因有关。
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