Ranjan Rajnish, Logette Emmanuelle, Marani Michela, Herzog Mirjia, Tâche Valérie, Scantamburlo Enrico, Buchillier Valérie, Markram Henry
Blue Brain Project, Ecole Polytechnique Fédérale de Lausanne, Geneva, Switzerland.
Laboratory of Neural Microcircuitry, Brain Mind Institute, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
Front Cell Neurosci. 2019 Aug 20;13:358. doi: 10.3389/fncel.2019.00358. eCollection 2019.
The voltage-gated potassium (Kv) channels, encoded by 40 genes, repolarize all electrically excitable cells, including plant, cardiac, and neuronal cells. Although these genes were fully sequenced decades ago, a comprehensive kinetic characterization of all Kv channels is still missing, especially near physiological temperature. Here, we present a standardized kinetic map of the 40 homomeric Kv channels systematically characterized at 15, 25, and 35°C. Importantly, the Kv kinetics at 35°C differ significantly from commonly reported kinetics, usually performed at room temperature. We observed voltage-dependent Q for all active Kv channels and inherent heterogeneity in kinetics for some of them. Kinetic properties are consistent across different host cell lines and conserved across mouse, rat, and human. All electrophysiology data from all Kv channels are made available through a public website (Channelpedia). This dataset provides a solid foundation for exploring kinetics of heteromeric channels, roles of auxiliary subunits, kinetic modulation, and for building accurate Kv models.
由40个基因编码的电压门控钾（Kv）通道可使所有电可兴奋细胞复极化，包括植物细胞、心肌细胞和神经元细胞。尽管这些基因在几十年前就已完成全序列测定，但对所有Kv通道的全面动力学表征仍然缺失，尤其是在接近生理温度的情况下。在此，我们展示了40种同源Kv通道在15°C、25°C和35°C下系统表征的标准化动力学图谱。重要的是，35°C下的Kv动力学与通常在室温下进行的动力学显著不同。我们观察到所有活性Kv通道的电压依赖性Q以及其中一些通道在动力学上的固有异质性。动力学特性在不同宿主细胞系中一致，并且在小鼠、大鼠和人类中保守。所有Kv通道的电生理数据都可通过一个公共网站（通道百科）获取。该数据集为探索异源通道的动力学、辅助亚基的作用、动力学调节以及构建准确的Kv模型提供了坚实基础。
