Kochalska Katarzyna, Łazorczyk Artur, Pankowska Anna, Dyndor Katarzyna, Kozioł Paulina, Stępniewski Andrzej, Pietura Radoslaw
Departament of Radiography, Medical University in Lublin, Poland.
Centrum ECO-TECH COMPLEX Maria Curie-Skłodowska University in Lublin, Poland.
Pol J Radiol. 2019 Apr 12;84:e190-e197. doi: 10.5114/pjr.2019.85147. eCollection 2019.
The aim of the work was to share the practical experience of preclinical and clinical proton magnetic resonance spectroscopy (HMRS) studies conducted using a 7-Tesla magnetic field strength scanner, taking into account the specificity of both settings in the context of translational research.
HMRS volunteer studies conducted using a Discovery 950 GE 7T scanner, were carried out with PRESS sequence, and a VOI measuring 2.0 × 2.0 × 2.0 cm placed in the white matter at the parietal occipital lobe. Rodent spectra obtained using a 7T Bruker were measured with PRESS, with a VOI 2.0 × 2.0 × 5.5 mm placed over the hippocampus.
HMRS data from humans and rats show that the brain spectra obtained in the same field are characterised by a similar neurochemical structure and spectral resolution. Spectra obtained from rats demonstrate the following metabolites: NAA, Glu, Gln, Ins, Cho, Cr, PCr, Tau, GABA, Lac, NAAG, and Asp. In turn, spectra from humans allowed estimation of the following metabolites: Ala, NAA, Glu, Gln, Ins, Cho, Cr, PCr, Tau, GABA, Lac, NAAG, and Asp. Signals from Gln, Glu with chemical shift around 2.4 ppm, from Cr, PCr, and GABA at 3 ppm, and signals from Cho and Tau at approximately 3.2 ppm, can be properly separated and estimated both in humans and in rats.
These results are promising in terms of broadening the knowledge of many neurological diseases by inducing them on animal models and then transferring this knowledge to clinical practice. In spite of this, important distinctions in the technical aspects and methodological differences of high-field HMRS in both preclinical and clinical conditions should be taken into account.
本研究旨在分享使用7特斯拉磁场强度扫描仪进行临床前和临床质子磁共振波谱（HMRS）研究的实践经验，同时考虑到转化研究背景下两种环境的特殊性。
使用Discovery 950 GE 7T扫描仪进行的HMRS志愿者研究采用PRESS序列，在顶枕叶白质中放置一个2.0×2.0×2.0 cm的感兴趣区（VOI）。使用7T布鲁克扫描仪获得的啮齿动物波谱采用PRESS测量，在海马体上方放置一个2.0×2.0×5.5 mm的VOI。
来自人类和大鼠的HMRS数据表明，在同一磁场中获得的脑波谱具有相似的神经化学结构和波谱分辨率。从大鼠获得的波谱显示出以下代谢物：N-乙酰天门冬氨酸（NAA）、谷氨酸（Glu）、谷氨酰胺（Gln）、肌醇（Ins）、胆碱（Cho）、肌酸（Cr）、磷酸肌酸（PCr）、微管相关蛋白tau（Tau）、γ-氨基丁酸（GABA）、乳酸（Lac）、N-乙酰天门冬氨酸谷氨酸（NAAG）和天冬氨酸（Asp）。反过来，来自人类的波谱可以估计以下代谢物：丙氨酸（Ala）、NAA、Glu、Gln、Ins、Cho、Cr、PCr、Tau、GABA、Lac、NAAG和Asp。在人类和大鼠中，化学位移约为2.4 ppm处的Gln、Glu信号，3 ppm处的Cr、PCr和GABA信号，以及约3.2 ppm处的Cho和Tau信号都可以得到适当的分离和估计。
这些结果对于通过在动物模型上诱导许多神经疾病并将这些知识转化为临床实践来拓宽对它们的认识很有前景。尽管如此，在临床前和临床条件下，高场HMRS在技术方面的重要差异和方法学差异仍应予以考虑。
