Söbbeler Franz Josef, Carrera Inés, Pasloske Kirby, Ranasinghe Millagahamada Gedara, Kircher Patrick, Kästner Sabine Beate Rita
Small Animal Clinic (Söbbeler, Kästner), University of Veterinary Medicine Hanover Foundation, Bünteweg 9, 30559, Hannover, Germany.
Clinic of Diagnostic Imaging (Carrera, Kircher), Vetsuisse Faculty, University of Zurich, Winterthurestrasse 258c, 8057, Zurich, Switzerland.
BMC Vet Res. 2018 Mar 5;14(1):69. doi: 10.1186/s12917-018-1396-1.
The purpose of this study was to determine the effects of isoflurane, sevoflurane, propofol and alfaxalone on the canine brain metabolite bioprofile, measured with single voxel short echo time proton magnetic resonance spectroscopy at 3 Tesla. Ten adult healthy Beagle dogs were assigned to receive isoflurane, sevoflurane, propofol and alfaxalone at 3 different dose rates each in a randomized cross-over study design. Doses for isoflurane, sevoflurane, propofol and alfaxalone were F'Iso 1.7 vol%, 2.1 vol%, 2.8 vol%, F'Sevo 2.8 vol%, 3.5 vol% and 4.7 vol%, 30, 45 and 60 mg kg h and 10, 15 and 20 mg kg h respectively. A single voxel Point Resolved Spectroscopy Sequence was performed on a 3 T MRI scanner in three brain regions (basal ganglia, parietal and occipital lobes). Spectral data were analyzed with LCModel. Concentration of total N-acetylaspartate (tNAA), choline, creatine, inositol and glutamine and glutamate complex (Glx) relative to water content was obtained. Plasma concentration of lactate, glucose, triglycerides, propofol and alfaxalone were determined. Statistics were performed using repeated measures ANOVA or Wilcoxon Sign Rank test with alpha = 5%.
Plasma glucose increased with isoflurane, sevoflurane and alfaxalone but decreased with propofol. Plasma lactate increased with all anesthetics (isoflurane > sevoflurane > propofol > alfaxalone). Cerebral lactate could not be detected. Only minor changes in cerebral metabolite concentrations of tNAA, choline, inositol, creatine and Glx occurred with anesthetic dose changes.
The metabolomic profile detected with proton magnetic resonance spectroscopy at 3 Tesla of canine brain showed only minor differences between doses and anesthetics related to tNAA, choline, creatine, inositol and Glx.
本研究旨在确定异氟烷、七氟烷、丙泊酚和阿法沙龙对犬脑代谢物生物图谱的影响，采用3特斯拉单像素短回波时间质子磁共振波谱法进行测量。在一项随机交叉研究设计中，将10只成年健康比格犬分为四组，每组分别接受三种不同剂量率的异氟烷、七氟烷、丙泊酚和阿法沙龙。异氟烷、七氟烷、丙泊酚和阿法沙龙的剂量分别为1.7体积%、2.1体积%、2.8体积%，2.8体积%、3.5体积%和4.7体积%，30、45和60毫克/千克/小时以及10、15和20毫克/千克/小时。在3T MRI扫描仪上对三个脑区（基底神经节、顶叶和枕叶）进行单像素点分辨波谱序列扫描。用LCModel分析光谱数据。获得相对于水含量的总N-乙酰天门冬氨酸（tNAA）、胆碱、肌酸、肌醇以及谷氨酰胺和谷氨酸复合物（Glx）的浓度。测定血浆中乳酸、葡萄糖、甘油三酯、丙泊酚和阿法沙龙的浓度。采用重复测量方差分析或Wilcoxon符号秩检验进行统计学分析，α=5%。
异氟烷、七氟烷和阿法沙龙使血浆葡萄糖升高，而丙泊酚使其降低。所有麻醉剂均使血浆乳酸升高（异氟烷>七氟烷>丙泊酚>阿法沙龙）。未检测到脑乳酸。随着麻醉剂量的变化，脑代谢物tNAA、胆碱、肌醇、肌酸和Glx的浓度仅发生轻微变化。
在3特斯拉下用质子磁共振波谱法检测的犬脑代谢组学图谱显示，与tNAA、胆碱、肌酸、肌醇和Glx相关的剂量和麻醉剂之间仅存在微小差异。
