Wu Xiaoxin, Showiheen Salah Ali A, Sun Antonia Rujia, Crawford Ross, Xiao Yin, Mao Xinzhan, Prasadam Indira
Department of Orthopedic Surgery, The Second Xiangya Hospital, Central South University, Changsha, Hunan, China.
Faculty of Science and Engineering , Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, QLD, Australia.
Methods Mol Biol. 2019;2054:81-91. doi: 10.1007/978-1-4939-9769-5_4.
Exosomes are small endosome-derived lipid nanoparticles (50-120 nm in diameter), actively secreted by exocytosis in most living cells. Recently, there is a growing interest of research focused on studying the exosome functions and to understand ways to use them for therapeutic applications in a wide variety of disorders, such as cancer, cardiovascular, neurodegenerative, and musculoskeletal diseases. Recently, a number of techniques have been developed for the isolation of exosomes such as ultracentrifugation, micro-filtration centrifugation, gradient centrifugation, and size-exclusion chromatography. In this chapter, we reveal the protocol and key insights into the isolation, purification, and characterization of exosomes using ultracentrifugation method.
外泌体是一种源自内体的小型脂质纳米颗粒（直径50-120纳米），由大多数活细胞通过胞吐作用主动分泌。最近，越来越多的研究关注于外泌体的功能，并探索将其用于多种疾病（如癌症、心血管疾病、神经退行性疾病和肌肉骨骼疾病）治疗应用的方法。最近，已经开发出多种用于分离外泌体的技术，如超速离心、微滤离心、梯度离心和尺寸排阻色谱法。在本章中，我们将揭示使用超速离心法分离、纯化和表征外泌体的方案及关键要点。
