通过多色荧光标记同时检测多种 DNA 损伤类型。
Simultaneous detection of multiple DNA damage types by multi-colour fluorescent labelling.
机构信息
Raymond and Beverly Sackler Faculty of Exact Sciences, School of Chemistry, Tel Aviv University, Tel Aviv 6997801, Israel.
出版信息
Chem Commun (Camb). 2019 Sep 19;55(76):11414-11417. doi: 10.1039/c9cc05198h.
Abstract
Herein we present an assay allowing concurrent detection of oxidative DNA damage and photoproducts. We apply DNA repair enzymes specific for each lesion type to incorporate spectrally distinct fluorescent nucleotides, enabling simultaneous quantification of the lesions on individual DNA molecules. We follow the repair of both damage types in skin cells exposed to artificial sunlight.
摘要
在此，我们介绍了一种能够同时检测氧化 DNA 损伤和光产物的分析方法。我们使用针对每种损伤类型的 DNA 修复酶来掺入具有独特光谱的荧光核苷酸，从而能够在单个 DNA 分子上同时定量检测损伤。我们在暴露于人工阳光下的皮肤细胞中研究了这两种损伤类型的修复。
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2
Long-read single-molecule maps of the functional methylome.长读单分子图谱的功能甲基组。
Genome Res. 2019 Apr;29(4):646-656. doi: 10.1101/gr.240739.118. Epub 2019 Mar 7.
3
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Curr Opin Biotechnol. 2019 Feb;55:30-35. doi: 10.1016/j.copbio.2018.08.001. Epub 2018 Aug 13.
4
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