Pinto-Ferreira Fernanda, Mitsuka-Breganó Regina, Monica Thais Cabral, Martins Felippe Danyel Cardoso, Matos Ricardo Luís Nascimento de, Mareze Marcelle, Nino Beatriz de Souza Lima, Narciso Simone Garani, Freire Roberta Lemos, Navarro Italmar Teodorico
Departamento de Medicina Veterinária Preventiva, Laboratório de Zoonoses e Saúde Pública, Universidade Estadual de Londrina - UEL, Londrina, PR, Brasil.
Departamento de Medicina Veterinária Preventiva, Laboratório de Parasitologia Veterinária, Universidade Estadual de Londrina - UEL, Londrina, PR, Brasil.
Rev Bras Parasitol Vet. 2019 Sep 2;28(3):518-521. doi: 10.1590/S1984-29612019044.
The objective of this study was to report an outbreak of human toxoplasmosis at a research institution in Londrina, Paraná, from December 2015 to February 2016. Blood samples from 26 symptomatic individuals were collected and the microparticle chemiluminescence immunoassay was performed to detect IgM, IgG and specific IgG avidity test in the official laboratory. A total of 20 people with symptoms and serology compatible with acute toxoplasmosis (IgM positive and IgG with low avidity) were selected as cases, while 45 asymptomatic employees working in the same teams and during the same shifts were selected as controls. All the participants of the investigation answered an epidemiological questionnaire. Three samples of water and one sludge from the institution's supply cisterns, 10 soil samples, 11 plant samples, three cat fecal samples and one domestic feline cadaver were collected for analysis of the polymerase chain reaction (PCR) for T. gondii. After analyzing the epidemiological data, the consumption of vegetables in the restaurant of the institution was the only variable associated with the occurrence of the disease. In laboratory results, all the samples showed negative results to PCR. The rapid recognition of the outbreak, early notification and investigation could have broken the chain of transmission early, thus preventing the emergence of new cases. In addition, the adoption of good food handling practices could have prevented the occurrence of the outbreak.
本研究的目的是报告2015年12月至2016年2月在巴拉那州隆德里纳的一个研究机构发生的一起人类弓形虫病疫情。采集了26名有症状个体的血样，并在官方实验室进行微粒化学发光免疫测定，以检测IgM、IgG和特异性IgG亲和力试验。共有20名有症状且血清学结果与急性弓形虫病相符（IgM阳性且IgG亲和力低）的人被选为病例，而45名在同一团队且同一班次工作的无症状员工被选为对照。所有调查参与者都回答了一份流行病学问卷。采集了该机构供水蓄水池的3份水样和1份污泥、10份土壤样本、11份植物样本、3份猫粪便样本和1具家猫尸体，用于进行弓形虫的聚合酶链反应（PCR）分析。在分析流行病学数据后，该机构餐厅的蔬菜消费是与疾病发生相关的唯一变量。在实验室结果中，所有样本的PCR检测均呈阴性。对疫情的快速识别、早期通报和调查本可及早打破传播链，从而防止新病例的出现。此外，采用良好的食品处理方法本可预防疫情的发生。
