Department of Clinical and Chemical Pathology, National Cancer Institute (NCI), Cairo University, Cairo, Egypt.
Medical Biochemistry and Molecular Biology, Cancer Biology Department, National Cancer Institute (NCI), Cairo University, Cairo, Egypt.
Andrologia. 2019 Nov;51(10):e13384. doi: 10.1111/and.13384. Epub 2019 Sep 4.
Prostate cancer (PC) is considered as the fifth cause of cancer deaths worldwide. The exact etiopathogenesis is unclear; however, genetic predisposition, hormonal influencers, lifestyle and environmental factors act as major contributors. It has been found that several miRNAs may play a crucial role in cancer initiation and progression. Here, in this study, we evaluated the peripheral blood levels of miR-21, miR-141, miR-221 and miR-18a expression among 80 prostate cancer patients (50 localised and 30 metastatic) and 30 benign prostatic hyperplasia patients compared to 50 normal control subjects, using RT-PCR. Our results of analysis of miR-21, miR-141, miR-18a and miR-221 in the plasma of PC patients showed that miR-18a is a powerful discriminator of PC patients from healthy controls as it had the highest AUC (0.966; 95% CI, 0.937-1.000), while miR-221 provided better differentiation of metastatic from localised PC (sensitivity was 92.9% at 100% specificity), and when we combine miR-18a and miR-221 for differentiating patients with MPC, it will increase the sensitivity to 96.4% at a specificity of 100% (AUC, 0.997; 95% CI, 0.988-1.0) (p < .000). This current study recommends that analysis of these miRNAs might have clinical value in enhancing PSA testing.
前列腺癌（PC）被认为是全球第五大癌症死亡原因。确切的病因学尚不清楚；然而，遗传易感性、激素影响、生活方式和环境因素是主要的致病因素。已经发现，几种 miRNA 可能在癌症的发生和发展中起关键作用。在这里，在这项研究中，我们使用 RT-PCR 评估了 80 名前列腺癌患者（50 名局限性和 30 名转移性）和 30 名良性前列腺增生患者与 50 名正常对照组之间外周血 miR-21、miR-141、miR-221 和 miR-18a 的表达水平。我们对 PC 患者血浆中 miR-21、miR-141、miR-18a 和 miR-221 的分析结果表明，miR-18a 是区分 PC 患者与健康对照者的有力鉴别因子，因为它具有最高的 AUC（0.966；95%CI，0.937-1.000），而 miR-221 则更好地鉴别转移性与局限性 PC（敏感性为 100%特异性时为 92.9%），当我们结合 miR-18a 和 miR-221 来区分 MPC 患者时，它将使敏感性提高到 100%特异性时的 96.4%（AUC，0.997；95%CI，0.988-1.0）（p<0.000）。本研究建议分析这些 miRNA 可能具有提高 PSA 检测的临床价值。
