Department of Clinical Laboratory, Kuma Hospital, Kobe, Hyogo 650-0011, Japan.
Department of Diagnostic Pathology and Cytology, Kuma Hospital, Kobe, Hyogo 650-0011, Japan.
Endocr J. 2019 Dec 25;66(12):1083-1091. doi: 10.1507/endocrj.EJ18-0348. Epub 2019 Sep 3.
Ancillary studies for primary nodal lymphomas have been well documented; however, studies of primary thyroid lymphoma (PTL) are limited. Here, we aimed to clarify the clinicopathological, flow cytometric, gene rearrangement, and karyotypic characteristics of PTL by investigation of a large series at a single institute. We performed flow cytometric, IgH rearrangement, and karyotypic analyses of 110 PTL tissues surgically resected at Kuma Hospital between January 2012 and April 2017. All PTLs were of B-cell origin, including mucosa-associated lymphoid tissue lymphoma (MALTL; 89 patients, 80.9%), diffuse large B-cell lymphoma (DLBCL; 18 patients, 16.4%), and follicular lymphoma (FL; three patients, 2.7%). In 96 (87.3%) patients, anti-thyroid antibodies were positive. For flow cytometry using aspirated and resected materials, light chain restriction was observed in 73.7% and 69.2% of examined cases, respectively. Heavy chain JH DNA rearrangement was observed in 65.4% of PTLs (58.1% of MALTL cases, 100% of DLBCL cases, and 100% of FL cases). Chromosomal abnormalities were detected in 49.0% of PTLs, and translocation was most frequently detected (24.0%), followed by addition (20.8%) and trisomy (18.8%). The most frequent (9.4%) karyotype was t(3;14)(q27;q32). Both FLs harbored t(14;18)(q32;q21), and the karyotype was not detected in patients with MALTL and DLBCL. The negative rate for all three examinations was 3.8%. We concluded that thyroid MALTL was cytogenetically different from that in other organs. Our results suggested that pre-operative flow cytometry analysis using aspirated materials was as reliable as that using resected materials.
辅助性研究在原发性淋巴结淋巴瘤中已有充分的记录；然而，原发性甲状腺淋巴瘤（PTL）的研究有限。在这里，我们旨在通过在单一机构进行的大型系列研究，阐明 PTL 的临床病理、流式细胞术、基因重排和核型特征。我们对 2012 年 1 月至 2017 年 4 月期间在久留米医院接受手术切除的 110 例 PTL 组织进行了流式细胞术、IgH 重排和核型分析。所有 PTL 均为 B 细胞起源，包括黏膜相关淋巴组织淋巴瘤（MALTL；89 例，80.9%）、弥漫性大 B 细胞淋巴瘤（DLBCL；18 例，16.4%）和滤泡淋巴瘤（FL；3 例，2.7%）。96 例（87.3%）患者的抗甲状腺抗体呈阳性。对于使用抽吸和切除材料进行的流式细胞术，分别有 73.7%和 69.2%的检查病例观察到轻链限制。65.4%的 PTL 存在重链 JH DNA 重排（58.1%的 MALTL 病例、100%的 DLBCL 病例和 100%的 FL 病例）。49.0%的 PTL 检测到染色体异常，最常见的是易位（24.0%），其次是添加（20.8%）和三体（18.8%）。最常见（9.4%）的核型是 t（3；14）（q27；q32）。两个 FL 均存在 t（14；18）（q32；q21），而 MALTL 和 DLBCL 患者的核型未检测到。三项检查的阴性率均为 3.8%。我们得出结论，甲状腺 MALTL 在细胞遗传学上与其他器官不同。我们的结果表明，使用抽吸材料进行的术前流式细胞术分析与使用切除材料一样可靠。
