Mathayan Manikannan, Jayaraman Selvaraj, Kulanthaivel Langeswaran, Suresh Arumugam
Centre for Drug Discovery and Development, Col. Dr.Jeppiaar Research Park, Sathyabama Institute of Science and Technology, Chennai.
Department of Biochemistry, Saveetha Dental College and Hospital, Chennai 77.
Bioinformation. 2019 Jul 31;15(7):506-512. doi: 10.6026/97320630015506. eCollection 2019.
Several antiviral compounds for HBV have been identified from traditionally used medicinal plants. We have earlier described the immune modulation properties of P. pinnata, a traditionally used Indian medicinal plant. Therefore, it is of interest to explore the anti-Hepatitis B virus activity of P. pinnata extracts by in-vitro screening assays. This study clearly demonstrated that the 5mg/ml concentration of the aqueous extract significantly inhibited the virus binding. Further, the spectral study was carried out for finding active compounds. The active chalcone derivatives namely, glabaarachalcone, and isopongachromene were isolated from P. pinnata aqueous seed extracts by standard spectral procedures. Virtual screening data shows that glabaarachalcone, and isopongachromene bound with HBV DNA polymerase protein target.
从传统药用植物中已鉴定出几种抗乙肝病毒的化合物。我们之前描述过印度传统药用植物羽叶金合欢的免疫调节特性。因此，通过体外筛选试验探索羽叶金合欢提取物的抗乙肝病毒活性具有重要意义。本研究清楚地表明，5mg/ml浓度的水提取物能显著抑制病毒结合。此外，还进行了光谱研究以寻找活性化合物。通过标准光谱程序从羽叶金合欢种子水提取物中分离出活性查尔酮衍生物，即光果甘草查尔酮和异蓬莪术色烯。虚拟筛选数据表明，光果甘草查尔酮和异蓬莪术色烯与乙肝病毒DNA聚合酶蛋白靶点结合。
