埃及肝细胞癌患者隐匿性乙型肝炎病毒感染。
Occult HBV infection among Egyptian hepatocellular carcinoma patients.
机构信息
Virology and immunology Unit, Cancer Biology Department, National Cancer Institute, Cairo University, 1st Kasr El-Aini St, 11197 Cairo, Egypt.
出版信息
Virol J. 2011 Mar 3;8:90. doi: 10.1186/1743-422X-8-90.
BACKGROUND
Occult HBV infection accelerates the progression of liver fibrosis, cirrhosis, and finally leading to hepatocellular carcinoma (HCC). This study analyzed the occult HBV-genotypes in HCC patients.
METHODS
To achieve our objective, matched serum and tissue samples were collected from 40 HCC patients. Three sets of primers were used for the HBV-DNA detection by nested-PCR, which cover the HBV-genome; Core, Surface and X genes. Genotyping system based on PCR using type-specific primers was applied on HBV-DNA positive samples.
RESULTS
Intrahepatic occult HBV-DNA was detected in 62.5%, whereas; Serum occult HBV-DNA were detected in only 22.5% of HCC patients. In patients' positive for both anti-HBs and anti-HBc, 10% had occult HBV in serum. In serologically negative HCV patients, 63% had intrahepatic HBV-DNA, and 21% had HBV-DNA in serum samples. HBV-genotype D (32%) and B (24%) attributed predominantly to intrahepatic HBV infections in HCC patients, whereas HBV-genotype A (4%) and C (8%) infections were the least observed.
CONCLUSION
This is the first study to show the genotypes of occult HBV infection in HCC Patients. We suggest that B or D may influence the outcome of HBV infection which may lead to the development of HCC.
背景
隐匿性乙型肝炎病毒（HBV）感染可加速肝纤维化、肝硬化的进展，最终导致肝细胞癌（HCC）。本研究分析了 HCC 患者隐匿性 HBV 基因型。
方法
为实现我们的目标，从 40 名 HCC 患者中采集了匹配的血清和组织样本。使用三套引物通过巢式 PCR 检测 HBV-DNA，该引物覆盖 HBV 基因组的核心、表面和 X 基因。基于 PCR 的基因分型系统应用于 HBV-DNA 阳性样本。
结果
62.5%的 HCC 患者检测到肝内隐匿性 HBV-DNA，而只有 22.5%的 HCC 患者检测到血清隐匿性 HBV-DNA。在抗-HBs 和抗-HBc 均阳性的患者中，有 10%的患者血清中有隐匿性 HBV。在 HCV 血清学阴性的患者中，63%的患者肝内有 HBV-DNA，21%的患者血清中有 HBV-DNA。HBV-D 型（32%）和 B 型（24%）主要归因于 HCC 患者的肝内 HBV 感染，而 A 型（4%）和 C 型（8%）感染则最少见。
结论
这是第一项研究显示 HCC 患者隐匿性 HBV 感染的基因型。我们认为 B 型或 D 型可能影响 HBV 感染的结局，从而导致 HCC 的发生。
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