Siriviriyakul Prasong, Chingchit Thidarat, Klaikeaw Naruemon, Chayanupatkul Maneerat, Werawatganon Duangporn
Alternative and Complementary Medicine for Gastrointestinal and Liver Diseases Research Unit, Department of Physiology, Faculty of Medicine, Chulalongkorn University, 1873 Rama 4 Road, Pathumwan, Bangkok, 10330, Thailand.
Department of Pathology, Faculty of Medicine, Chulalongkorn University, 1873 Rama 4 Road, Pathumwan, Bangkok, 10330, Thailand.
Heliyon. 2019 Aug 27;5(8):e02222. doi: 10.1016/j.heliyon.2019.e02222. eCollection 2019 Aug.
Curcumin, an active constituent of rhizomes of , exhibits a variety of biological activities such as anti-inflammation and anti-oxidant. The present study aims to examine the effects of curcumin on oxidative stress, inflammation and apoptosis in L-arginine induced acute pancreatitis (AP) in mice.
Male ICR mice were randomly divided into 4 groups. Control group received intraperitoneal injection (i.p.) of 1% DMSO as a vehicle. AP group received two doses of i.p. L-arginine (L-Arg) 450 mg/100 g body weight (BW) at 1-hour interval. AP plus low-dose curcumin group received i.p. curcumin 50 mg/kg BW 1 hour before L-Arg injection and then once daily for 3 days. AP plus high-dose curcumin group received i.p. curcumin 200 mg/kg BW 1 hour before L-Arg injection and then once daily for 3 days. All mice were sacrificed at 72 hours. Pancreatic tissue was obtained for histological evaluation, immunohistochemical studies for nuclear factor-kappa beta (NF-kβ), apoptosis and myeloperoxidase (MPO), and Western blot analyses for 4-Hydroxynonenal (4-HNE). Blood samples were collected for amylase analysis.
Mean body weight was significantly lower in AP group than in control group, while in curcumin group, body weight was maintained. The serum amylase, number of MPO positive cells, NF-kB positive cells, TUNEL positive cells, and 4-HNE expression significantly increased in AP group when compared with control group, but decreased in low and high-dose curcumin groups. Mice in AP group developed severe pancreatic inflammation, edema and fat necrosis. While mice in low and high-dose curcumin groups showed a significant improvement in histopathological scores. There was no significant difference between low and high doses of curcumin.
Curcumin could attenuate acute pancreatitis via anti-oxidant, anti-inflammation and anti-apoptosis property leading to the improvement in pancreatic damage.
姜黄素是姜黄根茎的一种活性成分，具有多种生物活性，如抗炎和抗氧化作用。本研究旨在探讨姜黄素对L-精氨酸诱导的小鼠急性胰腺炎（AP）氧化应激、炎症和细胞凋亡的影响。
雄性ICR小鼠随机分为4组。对照组腹腔注射（i.p.）1%二甲基亚砜（DMSO）作为溶剂。AP组间隔1小时腹腔注射两剂L-精氨酸（L-Arg），剂量为450 mg/100 g体重（BW）。AP加低剂量姜黄素组在注射L-Arg前1小时腹腔注射姜黄素50 mg/kg BW，然后连续3天每天注射一次。AP加中剂量姜黄素组在注射L-Arg前1小时腹腔注射姜黄素200 mg/kg BW，然后连续3天每天注射一次。所有小鼠在72小时后处死。获取胰腺组织进行组织学评估、核因子-κB（NF-κB）、细胞凋亡和髓过氧化物酶（MPO）的免疫组化研究，以及4-羟基壬烯醛（4-HNE）的蛋白质印迹分析。采集血样进行淀粉酶分析。
AP组的平均体重显著低于对照组，而姜黄素组的体重保持稳定。与对照组相比，AP组血清淀粉酶、MPO阳性细胞数、NF-κB阳性细胞数、TUNEL阳性细胞数和4-HNE表达显著增加，但在低剂量和高剂量姜黄素组中降低。AP组小鼠出现严重的胰腺炎症、水肿和脂肪坏死。而低剂量和高剂量姜黄素组小鼠的组织病理学评分有显著改善。低剂量和高剂量姜黄素之间无显著差异。
姜黄素可通过抗氧化、抗炎和抗凋亡特性减轻急性胰腺炎，从而改善胰腺损伤。
