Ragy Merhan M, Ali Fatma F, Toni Nisreen D M
Medical Physiology Department, Faculty of Medicine, Minia University, Minia, Egypt.
Pathology Department, Faculty of Medicine, Minia University, Minia, Egypt.
Endocr Regul. 2019 Oct 1;53(4):221-230. doi: 10.2478/enr-2019-0022.
Acute pancreatitis (AP) is a life-threatening condition. Using antioxidants in AP is insufficient and conflicting. Therefore, this study compared the effect of hydrogen sulfide (H2S) donor, sodium hydrosulfide (NaHS), leptin or curcumin pretreatment on AP induced by L-arginine.
Forty adult male rats were used and classified into: 1) control; 2) AP group [each rat was intraperitoneally (i.p.) injected with 2 doses of L-arginine of 250 mg/100 g body weight (b.w.) with an interval of 1 h]; 3) NaHS+AP group (each rat was i.p. injected with 10 mg/kg b.w. of NaHS 1 h before induction of AP); 4) leptin+AP group (each rat was pretreated with 10 μg/kg b.w. of leptin 30 min before induction of AP; and 5) curcumin+AP group (in which rats were i.p. injected with 150 mg/kg b.w. of curcumin 30 min before induction of AP). Serum amylase, lipase, nitric oxide (NO), tumor necrosis factor alpha (TNF-α), and corticosterone (CORT) levels were assayed. In addition, pancreatic tissues were obtained for histopathological examination and malondialde-hyde (MDA), total antioxidant capacity (TAC), and inducible nitric oxide synthase (iNOS) levels were measured.
All AP treated groups showed significant decrease in serum levels of pancreatic enzymes, NO, and TNF-α, and pancreatic MDA and iNOS levels, while TAC levels were significantly increased. NaHS caused more limitation of inflammation than leptin and curcumin by affecting iNOS. Leptin was more potent than curcumin due to the stimulatory effect of leptin on glucocorticoid release to counteract inflammation.
NaHS was more effective in AP amelioration than the leptin and curcumin.
急性胰腺炎（AP）是一种危及生命的疾病。在急性胰腺炎中使用抗氧化剂的效果并不充分且存在争议。因此，本研究比较了硫化氢（H₂S）供体硫氢化钠（NaHS）、瘦素或姜黄素预处理对L-精氨酸诱导的急性胰腺炎的影响。
使用40只成年雄性大鼠，分为：1）对照组；2）急性胰腺炎组[每只大鼠腹腔内（i.p.）注射2剂250 mg/100 g体重（b.w.）的L-精氨酸，间隔1小时]；3）NaHS+急性胰腺炎组（每只大鼠在诱导急性胰腺炎前1小时腹腔内注射10 mg/kg b.w.的NaHS）；4）瘦素+急性胰腺炎组（每只大鼠在诱导急性胰腺炎前30分钟用10 μg/kg b.w.的瘦素预处理）；5）姜黄素+急性胰腺炎组（大鼠在诱导急性胰腺炎前30分钟腹腔内注射150 mg/kg b.w.的姜黄素）。检测血清淀粉酶、脂肪酶、一氧化氮（NO）、肿瘤坏死因子α（TNF-α）和皮质酮（CORT）水平。此外，获取胰腺组织进行组织病理学检查，并测量丙二醛（MDA）、总抗氧化能力（TAC）和诱导型一氧化氮合酶（iNOS）水平。
所有接受治疗的急性胰腺炎组血清中胰腺酶、NO和TNF-α水平以及胰腺MDA和iNOS水平均显著降低，而TAC水平显著升高。NaHS通过影响iNOS比瘦素和姜黄素更能限制炎症。由于瘦素对糖皮质激素释放的刺激作用以对抗炎症，瘦素比姜黄素更有效。
NaHS在改善急性胰腺炎方面比瘦素和姜黄素更有效。
