Hemdan Bahaa A, El-Liethy Mohamed Azab, ElMahdy M E I, El-Taweel Gamila E
Environmental Microbiology Lab., Water Pollution Research Department, National Research Centre, Dokki, 12622, Giza, Egypt.
Environmental Virology Lab., Water Pollution Research Department, National Research Centre, Dokki, Giza, 12622, Egypt.
Heliyon. 2019 Aug 23;5(8):e02271. doi: 10.1016/j.heliyon.2019.e02271. eCollection 2019 Aug.
The bacterial profiles of natural household biofilm have not been widely investigated. The majorities of these bacterial lineages are not cultivable. Thus, this study aims (i) to enumerate some potential bacterial lineages using culture based method within biofilm samples and confirmed using Biolog GEN III and polymerase chain reaction (PCR). (ii) To investigate the bacterial profiles of communities in two biofilm samples using next generation sequencing (NGS). Forty biofilm samples were cultured and colonies of each selected prevailing potential lineages (, , , and ) were selected for confirmation. From obtained results, the counts of the tested bacterial lineages in kitchen biofilm samples were greater than those in bathroom samples. Precision of PCR was higher than Biolog GEN III to confirm the bacterial isolates. Using NGS analysis, the results revealed that a total of 110,554 operational taxonomic units (OTUs) were obtained for two biofilm samples, representing kitchen and bathroom biofilm samples. The numbers of phyla in the kitchen biofilm sample (35 OTUs) was higher than that in bathroom sample (18 OTUs). A total of 435 genera were observed in the bathroom biofilm sample compared to only 256 in the kitchen sample. Evidences have shown that the empirical gadgets for biofilm investigation are becoming convenient and affordable. Many distinct bacterial lineages observed in biofilm are one of the most significant issues that threaten human health and lead to disease outbreaks.
天然家庭生物膜的细菌谱尚未得到广泛研究。这些细菌谱系中的大多数不可培养。因此，本研究旨在：（i）使用基于培养的方法在生物膜样本中列举一些潜在的细菌谱系，并使用Biolog GEN III和聚合酶链反应（PCR）进行确认。（ii）使用下一代测序（NGS）研究两个生物膜样本中群落的细菌谱。培养了40个生物膜样本，并选择每个选定的主要潜在谱系（ 、 、 、 和 ）的菌落进行确认。从获得的结果来看，厨房生物膜样本中测试细菌谱系的数量高于浴室样本中的数量。PCR确认细菌分离株的精度高于Biolog GEN III。使用NGS分析，结果显示两个生物膜样本共获得110,554个可操作分类单元（OTU），分别代表厨房和浴室生物膜样本。厨房生物膜样本中的门数量（35个OTU）高于浴室样本（18个OTU）。浴室生物膜样本中总共观察到435个属，而厨房样本中只有256个。有证据表明，用于生物膜研究的经验工具正变得方便且经济实惠。在生物膜中观察到的许多不同细菌谱系是威胁人类健康并导致疾病爆发的最重要问题之一。
