一种用于确定溶瘤病毒诱导免疫原性肿瘤细胞死亡能力的功能测定法。

A Functional Assay to Determine the Capacity of Oncolytic Viruses to Induce Immunogenic Tumor Cell Death.

作者信息

Delaunay Tiphaine, Achard Carole, Grégoire Marc, Tangy Frédéric, Boisgerault Nicolas, Fonteneau Jean-François

机构信息

CRCINA, INSERM, Université d'Angers, Université de Nantes, Nantes, France.

Immunology Graft Oncology, Labex IGO, Nantes, France.

出版信息

Methods Mol Biol. 2020;2058:127-132. doi: 10.1007/978-1-4939-9794-7_8.

DOI:10.1007/978-1-4939-9794-7_8

PMID:31486035

Abstract

Oncolytic immunotherapy efficacy relies partially on the induction of immunogenic tumor cell death following infection with oncolytic viruses (OV) to induce an antitumor immune response. Here, we describe a method to determine if an OV is able to induce such an immunogenic tumor cell death. This method consists in testing whether tumor cells lysed by an OV are able to induce the maturation of human monocyte-derived immature dendritic cells (Mo-iDC).

摘要

溶瘤免疫疗法的疗效部分依赖于溶瘤病毒（OV）感染后诱导免疫原性肿瘤细胞死亡，以引发抗肿瘤免疫反应。在此，我们描述一种方法来确定一种OV是否能够诱导这种免疫原性肿瘤细胞死亡。该方法包括检测被OV裂解的肿瘤细胞是否能够诱导人单核细胞来源的未成熟树突状细胞（Mo-iDC）成熟。