miR-223-3p 通过靶向 SHOX2 抑制口腔鳞状细胞癌的增殖和转移。
MiR-223-3p inhibits proliferation and metastasis of oral squamous cell carcinoma by targeting SHOX2.
机构信息
Department of Periodontal Mucosa, Jinan Stomatological Hospital, Jinan, China.
出版信息
Eur Rev Med Pharmacol Sci. 2019 Aug;23(16):6927-6934. doi: 10.26355/eurrev_201908_18732.
OBJECTIVE
The aim of this study was to explore the role of microRNA-233-3p (miR-233-3p) in the development of oral squamous cell carcinoma (OSCC), and to elucidate the underlying mechanism.
PATIENTS AND METHODS
The expression of miR-233-3p in OSCC tissues and cell lines was detected by quantitative real-time polymerase chain reaction (qRT-PCR). The target of miR-233-3p was detected and evaluated by L-test and Western blot assays, respectively. Furthermore, the effects of miR-233-3p on cell proliferation, migration and apoptosis were discussed by cell counting kit-8 (CCK-8), scratch-wound and flow cytometry test.
RESULTS
MiR-233-3p was lowly expressed in OSCC tissues and cells. Short stature homeobox 2 (SHOX2) was predicted and verified as the downstream target gene of miR-233-3p. Inhibiting the expression of SHOX2 could significantly reduce the malignant behaviors of OSCC cells. The proliferation, migration and anti-apoptotic abilities of miR-233-3p overexpressed cells were obviously limited. However, the recovery of SHOX2 counteracted the beneficial effect of miR-233-3p.
CONCLUSIONS
MiR-223-3p acted as a tumor suppressor gene in OSCC by targeting SHOX2. Our findings revealed that miR-223-3p/SHOX2 axis could be a potential therapeutic target for OSCC.
目的
本研究旨在探讨微小 RNA-233-3p（miR-233-3p）在口腔鳞状细胞癌（OSCC）发展中的作用，并阐明其潜在机制。
方法
通过实时定量聚合酶链反应（qRT-PCR）检测 miR-233-3p 在 OSCC 组织和细胞系中的表达。通过 L 测试和 Western blot 分析分别检测和评估 miR-233-3p 的靶标。此外，通过细胞计数试剂盒-8（CCK-8）、划痕试验和流式细胞术试验探讨 miR-233-3p 对细胞增殖、迁移和凋亡的影响。
结果
miR-233-3p 在 OSCC 组织和细胞中低表达。矮小同源盒 2（SHOX2）被预测并验证为 miR-233-3p 的下游靶基因。抑制 SHOX2 的表达可显著降低 OSCC 细胞的恶性行为。miR-233-3p 过表达细胞的增殖、迁移和抗凋亡能力明显受限。然而，SHOX2 的恢复抵消了 miR-233-3p 的有益作用。
结论
miR-233-3p 通过靶向 SHOX2 在 OSCC 中发挥肿瘤抑制基因的作用。我们的研究结果表明，miR-233-3p/SHOX2 轴可能是 OSCC 的潜在治疗靶点。
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