Department of Burns, People's Hospital of Gansu Province, Lanzhou, China.
Eur Rev Med Pharmacol Sci. 2019 Aug;23(16):6991-6996. doi: 10.26355/eurrev_201908_18739.
Recent studies have revealed the crucial role of long non-coding RNAs (lncRNAs) in tumor progression. This study aims to identify the biological function of lncRNA OR3A4 in the progression of melanoma.
OR3A4 expression in melanoma cells and tissue samples was detected by Real Time-quantitative Polymerase Chain Reaction (RT-qPCR). The regulatory effects of OR3A4 on melanoma cells were identified by performing transwell assay and wound healing assay in vitro. The underlying mechanism of OR3A4 in mediating the progression of melanoma was explored by RT-qPCR and Western blot.
OR3A4 expression was remarkably upregulated in melanoma tissues compared with normal tissues. Moreover, migration and invasion of melanoma cells were inhibited after knockdown of OR3A4 in vitro, which were promoted after overexpression of OR3A4. Furthermore, the targeted proteins in phosphatidylinositol 3-kinase/protein kinase B (PI3K/AKT) signaling pathway were downregulated after knockdown of OR3A4 in vitro, which were upregulated by overexpressed OR3A4.
OR3A4 could promote the invasion and migration of melanoma cells by inducing the PI3K/AKT signaling pathway, which may offer a new therapeutic intervention for melanoma patients.
最近的研究揭示了长非编码 RNA（lncRNA）在肿瘤进展中的关键作用。本研究旨在确定 lncRNA OR3A4 在黑色素瘤进展中的生物学功能。
通过实时定量聚合酶链反应（RT-qPCR）检测黑色素瘤细胞和组织样本中的 OR3A4 表达。通过体外 Transwell 测定和划痕愈合试验鉴定 OR3A4 对黑色素瘤细胞的调节作用。通过 RT-qPCR 和 Western blot 探索 OR3A4 在介导黑色素瘤进展中的潜在机制。
OR3A4 在黑色素瘤组织中的表达明显高于正常组织。此外，体外敲低 OR3A4 后，黑色素瘤细胞的迁移和侵袭受到抑制，而过表达 OR3A4 则促进了迁移和侵袭。此外，体外敲低 OR3A4 后，磷脂酰肌醇 3-激酶/蛋白激酶 B（PI3K/AKT）信号通路中的靶向蛋白表达下调，而过表达 OR3A4 则上调了这些蛋白的表达。
OR3A4 可能通过诱导 PI3K/AKT 信号通路促进黑色素瘤细胞的侵袭和迁移，这可能为黑色素瘤患者提供新的治疗干预措施。
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