School of Engineering, China Pharmaceutical University, Nanjing, 211198, China.
State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, 210023, China.
Bull Environ Contam Toxicol. 2019 Oct;103(4):630-635. doi: 10.1007/s00128-019-02699-7. Epub 2019 Sep 5.
The widespread use of bisphenol S (BPS) as a bisphenol A substitute increases its potential of release into the aquatic environments. However, the degradation of BPS in aquatic systems is largely unknown, which will dictate its fate and toxicity. In this study, a bacterial consortium was enriched from river sediments and the dynamic changes of community structure during bacterial acclimation were studied. BPS degrading bacterial strains isolated from the consortium were identified by 16S rRNA analysis. The efficiency of the consortium and strains for BPS degradation were further evaluated. After 28 days of acclimation, the microbial diversity decreased significantly and four bacterial genera Hyphomicrobium, Pandoraea, Rhodococcus, and Cupriavidus with relative abundances of 5.1%-52.8% became dominant in the consortium. Total of two pure strains including Terrimonas pekingensis and Pseudomonas sp. were isolated from the consortium, using BPS as the sole carbon source. The consortium was highly efficient to degrade BPS, and 99% of BPS with an initial concentration of 50 mg/L was removed within 10 days at pH 7 and 30°C. In comparison with the consortium, a single strain cultures had lower BPS degradation efficiency. These findings indicate that BPS will degrade rapidly under aerobic conditions in river sediments and have implication for BPS-contaminated site remediation using the enriched consortium.
双酚 S（BPS）作为双酚 A 的替代品被广泛使用，增加了其向水生环境释放的可能性。然而，BPS 在水生系统中的降解情况在很大程度上尚不清楚，这将决定其命运和毒性。在这项研究中，从河流沉积物中富集了一个细菌混合体，并研究了细菌驯化过程中群落结构的动态变化。通过 16S rRNA 分析，从混合体中分离出的 BPS 降解细菌菌株进行了鉴定。进一步评估了混合体和菌株对 BPS 降解的效率。经过 28 天的驯化，微生物多样性显著下降，4 个细菌属 Hyphomicrobium、Pandoraea、Rhodococcus 和 Cupriavidus 的相对丰度为 5.1%-52.8%，成为混合体中的优势菌属。从混合体中分离到了两株纯菌，分别是恶臭假单胞菌和假单胞菌，它们都可以以 BPS 为唯一碳源生长。该混合体对 BPS 具有高效的降解能力，在 pH 值为 7 和 30°C 的条件下，初始浓度为 50mg/L 的 BPS 在 10 天内去除率达到 99%。与混合体相比，单一菌株培养物的 BPS 降解效率较低。这些发现表明，BPS 在河流沉积物的好氧条件下会迅速降解，这对于利用富集混合体修复 BPS 污染场地具有重要意义。
