Farias Julia de Morais, Argolo Leandro Araujo, Neves Raquel A F, Krepsky Natascha, Bitencourt José Augusto P
Graduate Program in Neotropical Biodiversity, Federal University of the State of Rio de Janeiro (UNIRIO), Rio de Janeiro, Rio de Janeiro, Brazil.
Laboratory of Water Microbiology (LACQUA), Department of Environmental Science, Institute of Biosciences, Federal University of the State of Rio de Janeiro (UNIRIO)Rio de Janeiro, Rio de Janeiro, Brazil.
PLoS One. 2025 Apr 24;20(4):e0320579. doi: 10.1371/journal.pone.0320579. eCollection 2025.
The continuous use of Di(2-ethylhexyl) phthalate (DEHP) in plastic products turns it into a ubiquitous contaminant in the environment. However, DEHP can cause harm to human beings, wildlife, and ecosystems due to its estrogenicity and toxicity. Thus, finding an efficient approach to removing this contaminant from the environment is crucial. The present study aimed to prospect and characterize a bacterial consortium (MP001) isolated from a neotropical mangrove for DEHP bioremediation. A laboratory experiment was performed with environmentally relevant DEHP concentrations (0.05, 0.09, 0.19, 0.38, 0.75, 1.50, 3.00, and 6.00 mg L-1) to determine the consortium resistance to this contaminant and high-throughput sequencing was accomplished to assess the bacterial composition, diversity, and potential ecological function of consortium MP001. The consortium MP001 presented a significant biomass increase throughout short-term incubations with increasing concentrations of DEHP (GLMs, p< 0.001). MP001 was constituted by Paraclostridium sp. (78.99%) and Bacillus sp. (10.73%). After 48 h of consortia exposure to DEHP, the bacterial population changed to Paraclostridium (50.00%), Staphylococcus sp. (12.72%), Staphylococcus epidermidis (10.40%) and Bacillus sp. (17.63%). In the negative control, the bacteria community was composed of Paraclostridium sp. (54.02%), Pseudomonas stutzeri (19.44%), and Staphylococcus sp. (11.97%). The alpha diversity of the MP001 consortium was not significant (Kruskall-Wallis; p > 0.05), and no significant difference was found between the DEHP treatment and the negative control. Furthermore, the potential ecological function found in the consortium MP001 with higher potential for application in bioremediation purposes was fermentation. The results found in this study highlight the potential of a bacterial consortium to be used in the bioremediation of DEHP-contaminated aquatic environments.
塑料制品中邻苯二甲酸二（2-乙基己基）酯（DEHP）的持续使用使其成为环境中一种普遍存在的污染物。然而，由于其雌激素活性和毒性，DEHP会对人类、野生动物和生态系统造成危害。因此，找到一种从环境中去除这种污染物的有效方法至关重要。本研究旨在对从新热带红树林分离得到的用于DEHP生物修复的细菌群落（MP001）进行勘探和表征。采用与环境相关的DEHP浓度（0.05、0.09、0.19、0.38、0.75、1.50、3.00和6.00 mg L-1）进行实验室实验，以确定该群落对这种污染物的耐受性，并通过高通量测序来评估MP001群落的细菌组成、多样性和潜在生态功能。在短期培养中，随着DEHP浓度的增加，MP001群落的生物量显著增加（广义线性模型，p < 0.001）。MP001由类梭状芽孢杆菌属（78.99%）和芽孢杆菌属（10.73%）组成。群落暴露于DEHP 48小时后，细菌种群变为类梭状芽孢杆菌（50.00%）、葡萄球菌属（12.72%）、表皮葡萄球菌（10.40%）和芽孢杆菌属（17.63%）。在阴性对照中，细菌群落由类梭状芽孢杆菌属（54.02%）、施氏假单胞菌（19.44%）和葡萄球菌属（11.97%）组成。MP001群落的α多样性不显著（Kruskal-Wallis检验；p > 0.05），DEHP处理组与阴性对照组之间未发现显著差异。此外，在MP001群落中发现的具有较高生物修复应用潜力的潜在生态功能是发酵。本研究结果突出了细菌群落用于DEHP污染水环境生物修复的潜力。
