Department of Orthopaedic Surgery, Sunnybrook Health Sciences Centre, University of Toronto, Toronto, ON, Canada.
Department of Neurological Surgery, Saint Louis University, St. Louis, MO, USA.
Adv Exp Med Biol. 2019;1169:243-256. doi: 10.1007/978-3-030-24108-7_13.
Heterogeneity among different subpopulations of human umbilical cord mesenchymal stem cell (hUCMSCs) lines is an ubiquitous phenomenon, with such variability being related to several factors including the identity of the individual donor, tissue source (Wharton's jelly vs. umbilical cord blood), culture conditions, as well as random variations in the cloning expansion process. In this chapter, we provide a general overview on the sources as well as available experimental techniques for proper identification of heterogeneity in hUCMSCs. Finally, we provide a brief discussion on the current scientific evidence regarding the potential superiority of subpopulations of hUCMSCs for specific clinical applications. Taking into account the exponential growth on the available experimental data on hUCMSCs in the past few years, this chapter is not intended to be comprehensive in nature, but rather is intended to provide a general overview about the central role which the topic of heterogeneity has in both basic science and clinical research in umbilical cord stem cells.
人脐带间充质干细胞（hUCMSCs）不同亚群之间的异质性是一种普遍现象，这种可变性与多个因素有关，包括个体供体的身份、组织来源（华通氏胶与脐带血）、培养条件以及克隆扩增过程中的随机变异。在本章中，我们提供了一个关于 hUCMSCs 异质性的来源以及可用的实验技术的概述。最后，我们简要讨论了关于 hUCMSCs 亚群在特定临床应用中具有潜在优势的当前科学证据。考虑到过去几年中关于 hUCMSCs 的可用实验数据呈指数级增长，本章并非旨在全面涵盖，而是旨在概述异质性在脐带干细胞的基础科学和临床研究中的核心作用。
